内蒙古地区刺叶柄棘豆(Oxytropis aciphylla Ledeb.)种群遗传多样性分析

Population Genetic Diversity Analysis of Oxytropis aciphylla Ledeb.of Inner Mongolia

用ISSR分子标记对内蒙古地区刺叶柄棘豆(Oxytorpis aciphylla Ledeb.)5个地理种群进行了种群遗传多样性分析。结果表明:刺叶柄棘豆种群具有较高的遗传多样性,11个ISSR引物扩增出215条带,总的多态位点百分率为98.14%,Shannon多样性指数I=0.2108,Nei基因多样性指数H=0.341 6,种内总基因多样性(Ht)为0.2108,种群内基因多样性(Hs)为0.160 4,大部分遗传变异(76.11%)的遗传变异存在于种群内,23.89%的遗传变异存在于种群间。遗传分化系数(Gst)为0.238 9,基因流(Nm)为1.592 9。5个居群间已有遗传分化趋势,遗传漂变不会引起遗传分化。UPGMA遗传距离聚类结果表明,5个地理种群中,植被类型为荒漠草原的4个种群之间遗传距离较近,与1个荒漠种群距离较远。

The genetic diversity of Oxytropis aciphylla Ledeb. was analyzed using inter simple sequence repeat（ISSR） markers. The results indicated that there was relatively high genetic diversity in Oxytropis aciphylla Ledeb. 215 bands were amplified from 5 natural populations by 11 ISSR primers. The percent of polymorphic loci was 98. 14%. The Shannon information index I was 0. 328 7, and Nei＇ s index H was 0. 197 2. The total gene diversity H, was 0. 210 8, and the gene diversity within populations Hs was 0. 160 4. 76.11% of genetic variation existed within populations while 23.89% of genetic variation existed among populations. The Gst was 0.238 9, and the gene flow was 1. 592 9. There was genetic vari- ation trend among populations. However the genetic drift would not cause the differentiation. The Nei＇ s UPGMA Dendrogram displayed that the genetic distance was close among 4 populations distributed in dessert steppe regions, while relatively far in a dessert population.