端粒酶在流感病毒诱导HeLa细胞凋亡中的作用

Effects of telomerase activity on HeLa cell apoptosis induced by influenza viruses

目的探讨端粒酶活性变化与流感病毒诱导HeLa细胞凋亡之间的关系。方法用A1亚型、B型及紫外线照射后的流感病毒分别感染HeLa细胞,在电镜下观察细胞形态,并用Annexin V-FITC染色流式细胞仪及PCR-ELISA法分别检测细胞凋亡诱导率与HeLa细胞端粒酶活性。结果B型、A1亚型流感病毒的凋亡诱导率最高达38.65%、23.94%(P<0.01),经紫外线照射后的流感病毒仍然具有一定的凋亡诱导能力;同时,HeLa细胞端粒酶活性均有所下降,其中B型流感病毒诱导后的HeLa细胞端粒酶活性下降比A1亚型更明显(P<0.01)。结论流感病毒降低肿瘤细胞的端粒酶活性,可能是其诱导肿瘤细胞凋亡的又一重要机制。

Objective To observe the correlation between telomerase activity and apoptosis in HeLa cells induced by influenza viruses. Methods HeLa cells were observed in the electron microscope after they had been infected by influenza viruses type A1, B and an UV exposed strain respectively. Besides, Annexin V-FITC staining flow cytometry （FCM） and PCR-ELISA were used to detect cell apoptosis and telomerase activity in HeLa cells respectively. Results The maximal rate of apoptotic cells induced by influenza virus type B and A1 were 38.65% and 23. 94% respectively （ P 〈 0.01 ）. The UV exposed influenza virus may have the ability to induce the apoptosis in HeLa cells. Furthermore, the telomerase activity in HeLa cells decreased. The decrease induced by influenza virus type B was more apparently than influenza virus type A1 （ P 〈 0.01 ）. Conclusion It may be another important mechanism of inducing apoptosis of turner cells that influenza viruses can decrease its telomerase activity.