摘要
目的观察人成纤维细胞注射移植后是否在裸鼠体内增殖并分泌胶原。方法将经原代培养的2×1010L-1人成纤维细胞1mL注射于BALB/CNU裸鼠真皮内,于1、2和3个月处死取材,行HE和Ⅰ、Ⅲ型胶原免疫组化染色。结果在1、2和3个月时可以观察到人成纤维细胞在裸鼠体内呈分裂象,随着时间的推移细胞外基质Ⅰ、Ⅲ型胶原分泌量呈增多趋势。结论人成纤维细胞注射移植裸鼠后,能够自行分裂、增殖并分泌人Ⅰ、Ⅲ型胶原,为自体成纤维细胞作为移植材料,发挥充填功能的持久性提供了理论依据。
Objective To investigate the proliferation and collagen secretion of transplanted human fibroblasts. Methods The solution containing human fibroblasts （2×10^10L^-1） was prepared and 1 mL was injected into the dermis of BALB/CNU nude mice. Animals were killed by the end of the 1st,2nd and 3rd month after injection. The dermis in the injected area was taken out and stained with HE. Immunohistochemical staining for type Ⅰ and type Ⅲ collagen was performed at the same time. Results Mitosis was observed by the end of the 1st,2nd and 3rd month. The concentration of type Ⅰ and type Ⅲ collagen in the extra cellular matrix increased with the passing of time. Conclusion Transplanted human fibroblasts can proliferate automatically in the dermis of nude mice and manufacture the type Ⅰ and type Ⅲ collagen in situ. long period of survival and secretion will make it possible for fibroblasts to become promising option to correct minimal tissue defects.
出处
《基础医学与临床》
CSCD
北大核心
2007年第10期1140-1145,共6页
Basic and Clinical Medicine
关键词
成纤维细胞
细胞移植
胶原
fibroblasts
cell transplantation
collagen
