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TO901317对海马神经元β-分泌酶表达及β-淀粉样肽生成的影响 被引量：1

The effect of TO901317 on the expression of β-secretase and β-amyloid peptide in hippocampal neuron
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摘要 目的研究肝X受体(LXR)配体TO901317对海马神经元淀粉样前体蛋白(APP)、α-分泌酶(ADAM10)和β-分泌酶(BACE1)mRNA表达的影响及与β-淀粉样肽(Aβ)生成的关系。方法大鼠海马神经元培养至第7天,在饲养液中加入2.0μmol/LTO901317,继续培养48h。应用RT-PCR方法研究海马神经元APP、ADAM10和BACE1等基因的mRNA的表达,放免法检测培养液Aβ含量的变化。结果TO901317降低海马神经元APP和BACE1 mRNA表达(P<0.01),减少培养液Aβ含量(P<0.01),但不影响ADAM10 mRNA表达(P>0.05)。结论TO901317激活LXR,能通过降低APP和BACE1的表达来减少海马神经元Aβ分泌。 Objective To investigate the effects of liver X receptor（LXR） ligand TO901317-LXR on the expression of β-amyloid precursor protein （APP） ,α-secretase （ADAM10） and β-secretase （ BACE1 ） in hippocampal neuron. Methods On the 7 th day ,hippocampal neurons were treated with 2.0 μmol/L TO901317. The expression of APP,ADAM10 and BACE1 mRNA were measured by RT-PCR and the content of Aβ in culture fluid was measured by radioimmunoassay method after cultured 48 h. Results TO901317 decreased the expression of APP and BACE1 mRNA （P 〈 0. 01 ） and the content of Aβ（ P 〈 0, 01 ）significantly, but had no effect on the expression of ADAM10 mRNA （ P 〉 0. 05 ）. Conclusions TO901317 could activate LXR and decrease the secretion of Aβ by decreasing the expression of APP and BACE1 mRNA in hippocampal neuron.
作者 张淑坤 姜玲玲 刘洁 杨荣慧 侯艳宁
机构地区 河北医科大学生物化学与分子生物学教研室 白求恩国际和平医院
出处 《中国老年学杂志》 CAS CSCD 北大核心 2007年第20期1953-1955,共3页 Chinese Journal of Gerontology
基金 国家自然科学基金(30371566) 河北省自然科学基金(303472)
关键词 肝X受体 TO901317 β-淀粉样肽前体蛋白 Β-分泌酶 Β-淀粉样肽 Liver X receptor TOg01317 β-amyloid precursor protein β-secretase β-amyloid peptide
分类号 R749.1 [医药卫生—神经病学与精神病学]
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参考文献12

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同被引文献7

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  • 6Koldamova RP, Lefterov IM,Staufenbiel M, et al. The liver X receptor ligand T0901317 decreases amyloid beta production in vitro and in a mouse model of Alzheimer's disease[ J ]. J Biol Chem,2005 ;280 (6) :4079-88.
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中国老年学杂志
2007年 第20期

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