MAT1-siRNA体外转染抑制胰腺癌细胞增殖和侵袭能力的研究

Inhibition of the proliferation and invasion of pancreatic cancer cells by MAT1 gene-targeted short interfering RNA in vitro

目的:观察siRNA沉默MAT1基因对胰腺癌细胞增殖和侵袭能力的影响,探讨MAT1基因作为胰腺癌基因治疗标靶的可行性。方法:在脂质体介导下将MAT1基因序列特异性siRNA体外转染人胰腺癌BxPC-3细胞,采用RT-PCR和W estern印迹法分析MAT1基因的表达,锥虫蓝染色计数法和Boyden小室模型分别测定细胞的增殖和体外侵袭能力变化。结果:与对照各组相比,实验组BxPC-3细胞MAT1 mRNA和蛋白在一定时间内均表达下调,其中mRNA表达在24 h和48 h分别下调达55.2%和64.3%(P<0.01);细胞体外增殖和侵袭能力均明显受抑,差异具统计学显著性(P<0.01)。结论:针对MAT1基因的siRNA可抑制胰腺癌细胞的增殖和侵袭能力,MAT1可能是胰腺癌基因治疗的靶点。

Objective ：To observe the influence of silencing MAT1 gene by short interfering RNA （siRNA） on the proliferation and invasion of human pancreatic cancer cells and discuss the feasibility of using MAT1 gene as therapeutic target for treatment of pancreatic cancer, nethods：BxPC-3 cells were transfected with sequence-specific siRNA targeting MAT1 gene with liposome mediation. The mRNA and protein expression of MATI were confirmed by RT-PCR and Western blot, respectively. The cell proliferation was measured by Trypan blue exclusion staining. The invasion ability was determined by Boyden chamber model in vitro. Results：The mRNA and protein expression of MAT1 were significantly down-regulated by siRNA in BxPC-3 cells compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% at 24 h and 48 h, respectively （P 〈0.01 ）. The cell proliferation and invasion ability of BxPC-3 cells were significantly inhibited in vitro（P 〈0.01 ）. Conclusion.The results suggest that siRNA targeting MAT1 gene inhibits the cell proliferation and invasion of BxPC-3 cells in vitro. MAT1 may be a potential target for gene therapy of human pancreatic cancer.