非小细胞肺癌中HPV感染与EGFR、VEGF表达的相关性研究

The correlation of HPV infection with the expression of epidermal growth factor receptor and vascular endothelial growth factor in non small cell lung cancer

目的:研究非小细胞肺癌(non-small cell lung cancer,NSCLC)中人乳头瘤病毒(human papillomavirus,HPV)感染与血管内皮生长因子(vascular endothelial growth factor,VEGF)和表皮生长因子受体(epidermal growth factor receptor,EGFR)表达的关系,探讨它们在肺癌发病中的作用。方法:采用PCR方法检测89例NSCLC和20例肺良性病变中HPV感染的情况;免疫组化SP法检测NSCLC的HPV(+)组和HPV(-)组中VEGF、EGFR分别表达的情况。结果:NSCLC组中HPV感染率(35/89)明显高于肺良性病变组(1/20)(P=0.007)。HPV(+)组VEGF和EGFR的表达均明显高于HPV(-)组,P值均<0.01。HPV(+)、EGFR(+)组和HPV(+)、EGFR(-)组中VEGF的表达无明显差异。HPV感染与肺癌组织的分化程度、淋巴结转移明显相关,而与组织学类型、吸烟、性别和年龄无关;VEGF的表达与肺癌的淋巴结转移明显相关,而与其他临床病理特征无明显相关性;EGFR的表达与各临床病理特征均无明显相关性。结论:HPV的感染与NSCLC的发生存在一定的相关性,HPV的感染可能通过促进VEGF、EGFR的表达,使肺癌易发生淋巴管生成,导致肺癌易通过淋巴结转移,预防HPV的感染对降低肺癌的发生及减少肺癌的早期转移可能有着重要的意义。

Objective..To study the relationship between human papillomavirus （HPV） infection and the expression of vascular endothelial growth factor receptor （VEGF） and epidermal growth factor （EGFR） in non small-cell lung cancer （NSCLC）, and discuss their significance in the oncogenesis of lung cancer. Methods：The PCR method was used to detect the HPV infection in 89 cases of NSCLC and 20 cases of benign pulmonary lesions. The immunohistochemicl SP method was used to measure the expressions of VEGF and EGFR in HPV （ ＋ ） and HPV （ - ） patients with NSCLC. Results：The infection rate of HPV in NSCLC group （35/89） was significantly higher than that in patients with benign pulmonary lesions （1/20, P =0.007）. The expression of EGFR and VEGF in HPV （ ＋ ） group was significantly higher than that in HPV （ - ） group （P 〈0.01 ）. The expression of VEGF had no significant difference between HPV（ ＋ ）, EGFR（ ＋ ） group and HPV（ ＋ ）, EGFR（ - ） group. The HPV infection significantly correlated with the differentiation degree and lymphatic metastasis of NSCLC, but did not correlate with the histological type, smoking, sex, and age of patients. The expression of VEGF was associated with the lymphatic metastasis but not with other clinicopathologic parameters. The expression of EGFR had no relationship with clinicopathologic features. Conclusion：HPV infection has a significant correlation with the oncogenesis of NSCLC. The HPV infection stimulates lymphangiogenesis and facilitated lymphatic metastasis of lung cancer by inducing the expression of EGFR and VEGF. Prevention of HPV infection has important significance to decrease the occurrence and reduce early metastasis of lung cancer.