环氧合酶-2在c9,t11-共轭亚油酸抑制肿瘤细胞转移中的作用

Study on inhibitory effects of c9,t11-conjugated linoleic acid on migration of human gastric carcinoma cell line via cyclooxygenase-2 pathway

目的研究 c9,t11-共轭亚油酸(c9,t11-CLA)抑制人胃腺癌细胞(SGC-7901)转移作用与亚油酸代谢途径中限速酶环氧合酶-2(COX-2)的关系。方法采用体外细胞培养方法,用 COX-2的选择性抑制剂 NS-398抑制 SGC-7901细胞中 COX-2的活性,再加入200、100、50和25 μmol/L 浓度的 c9,t11-CLA,利用重组基底膜侵袭实验、黏附实验、趋向运动实验检测 c9,t11-CLA 抑制肿瘤细胞转移的作用与 COX-2的关系。结果与 NS-398组比较,NS-398+100μmol/L c9,t11-CLA 和 NS-398+200 μmol/L c9,t11-CLA 对 SGC-7901细胞的侵袭有明显的抑制作用,穿过膜的细胞数分别为48.7±1.5(F=14.309,P=0.000)和43.7±4.0(F=19.005,P=0.000);NS-398+200 μmol/L c9,t11-CLA组能显著降低 SGC-7901细胞对基质成分层粘连蛋白、纤维粘连蛋白和基质胶的黏附作用,所测吸光度值(A 值)分别为0.052±0.011,0.058±0.008(F=3.063,P=0.021)和0.042±0.004(F=6.692,P=0.001;F=11.999,P=0.000);NS-398+200μmol/L c9,t11-CLA 对 SGC-7901细胞的趋向运动能力无明显的抑制作用(F=1.380,P=0.276),其中 NS-398+200μmol/L c9,t11-CLA 组细胞数为26.6±3.4。结论 c9,t11-CLA 具有抑制 SGC-7901细胞体外侵袭能力、黏附能力和趋向运动能力,这种抑制作用可能与 COX-2途径有关。

Objective To study the inhibitory effects of c9,tll-conjugated linoleic acid （c9,tll- CLA） on migration of human gastric carcinoma cell line （SGC-7901） via cyclooxygenase-2 （COX-2） pathway. Methods After inhibiting COX-2 activity by 100 p.mol/L COX-2 inhibitor NS-398 in SGC-7901 cell, we treated SGC-7901 cells with c9, tl 1-CLA at a concentration of 200,100,50,25μmol/L for 24 h, respectively. Using reconstituted basement membrane invasion, adhesion, chemotaxis assays, we detected the effect of c9, tl 1-CLA and COX-2 on the cell migration. Results Compared to NS-398 group, 200, 100μmol/L c9,tll-CLA significantly suppressed SGC-7901 cells invading into the reconstituted basement membrane（F = 14. 309, P = 0. 000; F = 19. 005, P = 0. 000） . 200 μmol/L c9, t11-CLA significantly inhibited SGC-7901 cells adhering to laminin, fibronectin and Matrigel （F = 3. 063, P = 0. 021 ;F = 6. 692, P = 0. 001 ; F = 11. 999, P = 0. 000 ）. The chemotaxis of SGC-7901 cells and inhibitory frequency were significantly decreased in the 200μmol/L c9 ,tll-CLA group（F = 1. 380,P =0. 276）. Conclusion c9,t11- CLA inhibits invasion, adhesion and chemotaxis of SGC-7901 cells, and the COX-2 plays an important role in the process.