胰岛素样生长因子-2对子宫颈癌细胞表面钾氯离子协同转运子表达的影响

Regulative function of insulin-like growth factor-2 on the expression of KCL cotransporter in Caski cell

目的研究子宫颈癌Caski细胞株中,胰岛素样生长因子-2(IGF-2)刺激细胞增殖的同时,对钾氯离子协同转运子(kcc)基因表达的调节作用。方法2006年1月至8月于中国医科大学盛京医院院中心实验室,采用酶联免疫吸附实验法确定不同时间点IGF-2细胞增殖曲线,采用聚合酶链反应法比较不同浓度、不同时间IGF-2作用下kcc1、kcc3、kcc4 mRNA表达水平的差异。结果低浓度的IGF-2刺激Caski细胞增殖的同时抑制细胞表面kcc基因的表达,kcc1、kcc3、kcc4 mRNA分别下降30%、50%和80%,随着IGF-2浓度的升高,对kcc基因的作用逐渐减弱。结论IGF-2对Caski细胞表面的kcc基因表达存在影响,这种刺激作用呈时间和浓度依赖性。为进一步研究IGF2通过kcc参与子宫颈细胞恶变过程提供了实验依据。

Objective To investigate the regulative function of insulin-like growth factor- 2 （IGF-2） on the expression of KCL cotransporter mRNA in Caski cell. Methods Make the growth curves of IGF-2 with Caski by MTT method in central labortary of Shengjing Hospital of China Medical University from January to August in 2006. Use IGF-2 to stimulate Caski cell with different concentrations and at different time points,then confirm the expression of kcc1 ,kcc3 .kcc4 genes by PCR. Result The expressions of kcc1 .kcc3.kcc4 mRNA decreased under the effect of IGF-2, with the inhibition rates of 30% .50% and 80%, respectively. The function became worse when the concentration of IGF-2 became higher. Conclusion The findings demonstrate that IGF-2 can restrain kcc mRNA expressions in a concentration- and time-dependent manner. These data provides the link of IGF-2 and kcc with the proliferation and invasion of cervical cancer.