低压缺氧复合NaCN中毒对大鼠呼吸频率及BALF成份的影响

Effects of NaCN intoxication and hypobaric hypoxia on respiration rate and BALF content in rats

目的研究低压缺氧复合氰化钠(NaCN)中毒对大鼠呼吸频率及支气管肺泡灌洗液(BALF)细胞数和总蛋白(TP)含量的影响。方法SD大鼠84只,随机分为常压常氧和低压缺氧2组。常压常氧组动物于重庆地区常规实验室内进行实验。将低压缺氧组动物置低压舱61 kPa环境放置72 h后开始实验。以3.6 mg/kg氰化钠皮下注射染毒,用RM6240型多道生理信号采集处理系统记录呼吸频率。制备BALF,测定BALF中细胞数,Lowery法测TP含量。结果低压缺氧致动物呼吸频率加快,但无统计学意义。BALF中细胞数和TP含量显著升高。低压缺氧动物NaCN中毒后呼吸频率及BALF中细胞数和TP含量进一步升高,均有显著性差异。结论低压缺氧加重NaCN中毒对大鼠呼吸系统的损伤作用。

Objective To evaluate the effects of NaCN intoxication and hypobaric hypoxia on respiration rate and BALF content in rats. Methods Tatolly 84 rats were randomized into the groups of non-hypoxia （NH） and hypobaric hypoxia （HH）. The animals in NH and HH groups were treated in the general laboratory and the hypobaric hypoxia chamber, respectively. The animals were administrated sub- cutaneously with 3.6 mg/kg sodium cyanide （NaCN）. Rat respiration rate was recorded with a RM6240 type electrophysiological machine. The bronchoalveolar lavage fluid （BALF） was collected. Cells count and total protein were measured. Results The respiration rate was not significantly increased by hypobaric hypoxia itself, but by cyanide intoxication, however. An obvious increasing of BALF cell count and total protein was determined in the hypoxia-treated animals. Cyanide intoxication further rose the levels of the BALF content. Conclusion Sodium cyanide-induced injuries of respiration system can be further increased by hypobaric hypoxia.