黄芩苷磷脂复合物抗炎时效初步研究

Study on the Time-Effect Relationship of Phytosomes of Baicalin in Anti-inflammatory Effect

目的比较黄芩苷、黄芩苷磷脂复合物的抗炎时效关系。方法制备黄芩苷磷脂复合物;用二甲苯致小鼠腹部皮肤毛细血管通透性亢进炎症模型和耳肿模型;分光光度法测定腹部蓝染皮肤伊文思蓝渗出量。结果抗炎时效曲线显示黄芩苷磷脂复合物组小鼠腹部皮肤伊文思蓝的渗出量在0.5 h即与对照组有显著差异,而黄芩苷则发生在1 h左右。磷脂复合物组3 h抗炎作用达到最强,21 h内均呈现出明显的抗炎作用。而黄芩苷组在4 h时抗炎作用最大,之后抗炎作用下降,16 h后已无作用。黄芩苷组与黄芩苷磷脂复合物组在给药后2 h抗炎作用已经表现出显著的差异,磷脂复合物组强于黄芩苷组。结论黄芩苷磷脂复合物的抗炎活性明显强于黄芩苷,且作用出现更迅速、维持时间更久。

OBJECTIVE To compare the time-effect relationship of Baicalin and Phytosomes of Baicalin in anti-inflammatory effect.METHODS The Phytosomes of Baicalin was prepared.The inflammation model of sthenic capillary vessel permeability was induced by xylene on venter skin of the mouse,and the model of otic swelling was induced by xylene on the mouse,respectively.The effusion quanitity of the E.wliwas of blue skin was measured by spectrophotogrophy.RESULTS The anti-inflammation time-effect curve showed that the effusion amount of the E.wliwas from mouse＇s venter skin in Phytosomes of Baicalin＇s group had statistical difference in 0.5h comparing with that of control group.However,This is occured after 1h in Baicalin group.Phytosomes of Baicalin reached the most anti-inflammatory effect at 3h,and kept the clearly effect within 21h.However,Baicalin reached the most anti-inflammatory effect at 4h,and then reduced,the effect was disappeared after 16h.Compared with the Baicalin,Phytosomes of Baicalin had statistical difference by orally administrated at 2h.CONCLUSION Compared to Baicalin,Phytosomes of Baicalin had stronger anti-inflammation effect which appeared faster and kept longer.