河南北柴胡HPLC指纹图谱的研究

Study On the HPLC Fingerprints of Bupleurum chinense from Henan

目的对河南产北柴胡的HPLC指纹图谱进行研究,建立河南北柴胡药材的质量评价体系。方法采用HPLC测定了河南产北柴胡及其同属品种共37批样品的指纹图谱。色谱条件:Hypersil C18柱(200 mm×4.6 mm,5μm),流动相为乙腈-水线性梯度洗脱,流速1 mL.min-1,检测波长为205,250 nm,柱温35℃。结果该判别方法准确可控,具有较好的实用性。结论所得河南北柴胡HPLC指纹图谱及其评价指标为科学评价河南北柴胡药材的质量提供了依据。

OBJECTIVE To study the HPLC fingerprint of Bupleurum chinense DC.from Henan Province and establish the quality evaluation system for this herb.METHODS HPLC method was used to analysis 37 samples of B.chinense DC.and it＇s sibling plants.Chromatographic condition： Hypersil C18 column（200 mm×4.6 mm,5 μm）,mobile phase is linear gradient elute with acetonitrile-water,1 mL·min-1 flow rate,205 nm,250 nm detection wave-length,35 ℃ column temperature. RESULTS This evaluation method is accurate and feasible.CONCLUSION The acquired HPLC fingerprints of B.chinense DC.and it＇s evaluate guide line provide the scientific quality assessment of B.chinense DC.