去除外膜的外周神经移植对大鼠脊髓损伤的影响

Effect of removed epineurium of peripheral nerve and transplanted into spinal cord injury in rats

目的检测大鼠脊髓损伤经显微外科技术处理的自体外周神经移植后神经纤维的再通及后肢功能恢复情况。方法利用改良Allen撞击方法建立脊髓打击损伤模型,12周后,将大鼠分为2组,实验组切取后肢腓肠神经,利用显微外科技术去除神经外膜,将其修剪成小段,游离移植于脊髓损伤处,对照组仅显露脊髓损伤段,不作神经移植。分三个时相点(1、2、3个月)进行辣根过氧化物酶(HRP)逆行示踪,并观察损伤移植处的形态学变化及大鼠后肢运动功能恢复情况。结果损伤移植处脊髓的形态学明显好转;损伤移植处上段脊髓中,移植1个月组有HRP阳性标记物,以后逐渐增多;移植组大鼠后肢运动功能明显恢复。结论经显微外科技术预处理外周神经组织移植后可促进大鼠脊髓结构和功能的恢复。

Objective To investigate the nerve recanalization and the motor function of hind legs after transplantation of peripheral nerve grafts treated with microsurgical technique at chronic spinal cord injury （SCI） in rats. Methods The SD rats were established into SCI model with improved Allen method. The rats were divided into two parts 12 weeks after the injury. In experimental group： by microsurgical technique. the sural nerves were removed epineurium and transplanted into SCI lesion, control group rats were treated without any operation. Retrograde HRP tracing through sciatic nerve were practiced at 1 month, 2 month, 3 month after transplantation of peripheral nerve grafts. The morphological changes were observed at section of spinal cord and the motor functions of both hind legs of rat were detected. Results The morphology of the injured spinal cord sections turned better. Retrograde HRP tracing through sciatic nerve showed some HRP positive markers at the site of near rostral end of the nearly injured part at one month after transplantation and increased with the time going by. Motor function of hind legs of rats recovered significantly in transplantation groups. Conclusion Peripheral nerve grafts treated with microsurgical technique have repairing effect on chronic spinal cord injury in rats.