HIF-1α VEGF Survivin蛋白在子宫内膜癌中的表达

Expression of HIF-1α,VEGF and Survivin in Endometrial Carcinoma and Its Clinical Significance

目的研究低氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)及凋亡抑制因子(Survivin)在子宫内膜癌中的表达及其意义,并探讨与Survivin HIF-1α及VEGF表达之间的关系。方法应用免疫组织化学SP方法检测HIF-1α、VEGF、Survivin在40例子宫内膜癌、22例子宫内膜复杂性增生及18例子宫内膜单纯性增生组织中的表达情况。结果HIF-1α、VEGF、Survivin在子宫内膜单纯性增生、子宫内膜复杂性增生及子宫内膜癌组织中阳性表达率依次增高,三组比较具有统计学意义(P<0.05)。子宫内膜癌组织中HIF-1α与VEGF蛋白表达呈显著正相关(r=0.469,P<0.01);Survivin与VEGF蛋白的表达呈显著的正相关(r=0.452,P<0.01);HIF-1α与Survivin的表达无显著相关性(r=0.209,P>0.05)。结论子宫内膜癌组织中HIF-1α、VEGF、Survivin蛋白参与了子宫内膜癌的发生发展。子宫内膜癌组织的HIF-1α、Survivin蛋白表达与VEGF呈正相关,Survivin蛋白与HIF-1α的表达无相关性,提示HIF-1α与Survivin可能通过上调VEGF蛋白的表达,抑制肿瘤细胞的凋亡和促进肿瘤的血管生成而促进子宫内膜癌的发生及发展。

Objective To study the expression of hypoxiainducible factor - 1 alpha （ HIF - 1α）, vascular endothelial growth factor （VEGF） and apoptosis inhibited factor（Survivin） in endometrial carcinoma and to investigate the relationship between HIF- 1α, VEGF and Survivin. Methods Immunohistochemistry method was used to detect the expression of HIF- 1α,VEGF and Survivin protein in 40 cases of endometrial adenocarcinoma, 22 cases of comlex hyperplasia of endometrium and 18 cases of simplex hyperplasia endometrium. Results HIF - 1α, VEGF and Survivin were expressed in endometrial adenocarcinoma tissues, and the positive rates were increased gradually in simple hyperplasia, complex hyperplasia and endometrial carcinoma. The expression of HIF - 1α was positively associated with VEGF （ r = 0. 469, P 〈 0. 01 ） , The expression of Survivin was positively associated with VEGF（ r =0. 452, P 〈 0.01 ）, but there was no correlation between HIF- 1α and Survivin（ r = 0. 209, P 〉 0. 05 ）. Conclusion The overexpression of HIF - 1α, VEGF and Survivin may play an important role in the carcinogenesis of endometrial carcinoma. The expression of HIF - 1α, Survivin is positively associated with VEGF, but there is no relationship between HIF-1α and Survivin. The overexpression of HIF - 1α and Survivin protein induces the tumor angiogenesis by activating the transcription of VEGF gene. They play important roles in the carcinogenesis of endometrial carcinoma.