系统性红斑狼疮患者热休克蛋白90和白细胞介素18的表达及意义

Expression of Heat Shock Protein 90 and Interleukin-18 in Patients With Systemic Lupus Erythematosus and Its Clinical Significance

目的:研究系统性红斑狼疮(SLE)患者热休克蛋白90(HSP90)和血浆白细胞介素18(IL-18)的表达水平及与疾病活动的关系。方法:运用Westernblot技术检测SLE患者外周血单个核细胞HSP90的表达,ELISA方法检测血浆中的IL-18水平,并与SLE疾病活动指数(SLEDAI)进行相关性分析。结果:⑴SLE患者HSP90的表达水平在活动期组(0.82±0.10)和稳定期组(0.54±0.09)与正常对照组(0.37±0.11)比较均具有显著性差异(分别P<0.01),且活动期组较稳定期组增高更明显(P<0.01);⑵SLE患者血浆IL-18水平在稳定期组(327.82±101.45pg/ml)和活动期组(459.79±134.08pg/ml)均显著高于正常对照组(252.32±76.45pg/ml)(分别为P<0.05,P<0.01);⑶SLE患者的HSP90和IL-18水平与SLEDAI评分之间呈正相关(分别为r=0.80,P<0.01;r=0.49,P<0.01)。结论:SLE患者外周血单个核细胞HSP90和血浆IL-18水平均显著增高,并且与SLE的病情活动密切相关,可能在SLE的发病机制中发挥重要作用。

Objective：To investigate the roles of heat shock protein 90（HSP 90）and Interleukin-18（IL-18）in patients with systemic lupus erythematosus （SLE）. Methods： HSP 90 was detected in peripheral blood mononuclear celIs（PBMC） by Western blot assay and ELISA was used to measure the plasma level of IL-18 from SLE patients and normal controls. The correlation analysis was performed between HSP 90, IL-18 and SLE disease activity index （SLEDAI）. Results： ①There was increased expression of HSP 90 in the SLE patients. The level of HSP 90 in active SLE group （0. 82 ± 0. 10） were higher than that in stable SLE group（0. 54 ±0. 09） （P〈0. 01 ）. HSP 90 in both SLE groups compared with that of normal control group（0.37 ± 0. 11）shown statistical significance （P〈0. 01 ）. ②Plasma level of IL-18 exhibited a significant increase in the stable SLE group（327. 82 ± 101.45 pg/ml） and active SLE group（459. 79 ± 134.08 pg/ml）compared with those in normal control group（252. 32 ± 76.45 pg/ml） （ P〈0. 05, P〈0. 01, respectively）. ③Levels of HSP90 and IL-18 in SLE patients were significantly positive correlated with SLEDAI scoring（r = 0. 80, P〈0. 01 ;r = 0. 49, P〈0. 01 ; respectively）. Conclusion： Increased levels of HSP 90 and IL-18 in SLE patients correlated with SLEDAI, it might play an important role in the pathogenesis of SLE.