外源性一氧化碳对小肠缺血再灌注大鼠血中碳氧血红蛋白的影响

Effect of exogenous carbon monoxide on carboxyhemoglobin blood concentration during intestinal ischemia/reperfusion in rats

目的:通过检测大鼠血中碳氧血红蛋白(COHb)浓度的变化,探讨小肠缺血再灌注过程中外源性应用不同浓度一氧化碳的安全性.方法:♂Wistar大鼠32只,随机分为假手术对照组(Ⅰ组)、小肠缺血再灌注组(Ⅱ组)及缺血时吸入浓度分别为100、250μL/L一氧化碳(CO)的Ⅲ、Ⅳ组;制作小肠缺血再灌注模型.分别于缺血前15 min(T_1),缺血后1 h(T_2),再灌注后1 h(T_3)、2 h(T_4)取动脉血测定COHb浓度.结果:Ⅰ组及Ⅱ组缺血再灌注前后各时点COHb浓度无明显差异,Ⅲ、Ⅳ组缺血后的T_2(4.1%±1.3%,10.1%±3.0%vs 1.0%±0.2%)、T_3(5.8%±1.1%,13.1%±2.8%vs 1.2%±0.3%)、T_4(6.8%±1.2%,13.3%±3.1%vs1.1%±0.2%)时点与Ⅱ组相应时点比较,COHb浓度升高(P<0.05).结论:大鼠小肠缺血再灌注过程中外源性应用100μL/L和250μL/L的CO均是安全的.

AIM： To evaluate the safety of exogenous carbon monoxide inhaled during intestinal ischemia/ reperfusion （IR） in rats, and to monitor its effect on carboxyhemoglobin （COHb） blood concentration. METHODS： Thirty-two male Wistar rats weighing 220-260 g were randomly divided into four groups of eight rats each： group Ⅰ, sham operation （control group）; group Ⅱ, small intestinal IR; group Ⅲ, 100 μL/L carbon monoxide （CO） inhalation; and group Ⅳ, 250 μL/L CO inhalation. Superior mesenteric artery （SMA） was isolated and clamped for 60 min, followed by 120 min reperfusion. In groups Ⅲ and Ⅳ, different doses of CO were continuously inhaled from the time of the SMA being clamped to the end of the experiment. In groups Ⅰ and Ⅱ, air was inhaled instead of CO. Blood samples were taken before the SMA was ischemia （T1）, 1 h （T2） clamped, at 15 min before after ischemia, and 1 h （T3）and 2 h （T4） after reperfusion, for determination of COHb blood concentrations. RESULTS： The COHb blood concentration increased significantly after CO inhalation in groups Ⅲ and Ⅳ when compared with group II at T2, T3 and T4 （4.1% ± 1.3% and 10.1% ± 3.0% vs 1.0± 0.2%; 5.8%± 1.1% and 13.1%± 2.8% vs 1.2% ± 0.3%; 6.8 ± 1.2 and 13.3% ±3.1% vs 1.1%± 0.2%; P 〈 0.05）. CONCLUSION： It is safe to inhale 100 μL/L and 250 μL/L CO during intestinal IR in rats.