榄香烯联合化疗治疗荷瘤小鼠

Therapeutic effects of elemene and chemotherapy in tumor-bearing mice

目的:观察榄香烯结合化疗药对荷瘤小鼠的治疗作用,为临床肿瘤的治疗提供理论依据.方法:构建荷瘤小鼠动物模型。肿瘤内注射给药,用逆转录聚合酶链式反应(RT-PCR)方法测其多药耐药基因(multidrugs resistance gene,mdr1)的表达,同时观察抑瘤率、肿瘤坏死情况和小鼠死亡率.结果:荷瘤组(LT)、化疗组(C)、榄香烯组(E)、化疗药+榄香烯组(CE)的mdrl的表达分别为(100%、100%、16.67%、8.33%),其中E、CE与LT组的mdr1比较有显著性差异(P<0.05);C、E、CE组抑瘤率(24.3%、29.7%、39.5%)和死亡率(20%、10%、10%)分别与LT组(0、40%)比较有显著性差异(P<0.05).病理显示,CE组肿瘤坏死明显,大体见肿瘤腐肉状,色晦暗,质软,小鼠生存活跃.结论:榄香烯可降低mdr1的表达并有良好的抗肿瘤的作用,与化疗药合用抗肿瘤作用更佳.

AIM： To explore a potential treatment for carcinoma by observing the therapeutic effects of elemene and chemotherapy in tumorearing mice （LT）. METHODS： Pharmaceuticals were injected into the centers of tumors in tumor-bearing mice, and the expression of the multidrug resistance gene （mdr1） was determined by polymerase chain reaction. reverse transcriptionpolymerase chain reaction. The rate of inhibition of tumor growth, the pathology of the tumor and mortality were noted. RESULTS： The expression levels of mdrl in chemotherapy （C）, elemene （E） and chemiotherapy＋elemene （CE） groups were 100%, 16.67% and 8.33% of the levels in the tumor-bearing （LT） group, respectively, and there was statistical significance between the E （or CE）and LT groups （P 〈 0.05）. The rates of inhibition of tumor growth in mice in groups C, E and CE were 24.3%, 29.7%, and 39.5%, respectively, and there was statistical significance when compared with LT mice, P 〈 0.05. The mortality of mice in groups C, E and CE was 20%, 10%, and 10%, respectively, and there was statistical significance when compared with LT mice （40%） （P 〈 0.05）. Tumor necrosis was outstanding in CE mice, and these mice were more energetic than the mice in other groups. CONCLUSION： Elemene can reduce the expression of the mdrl gene and is effective in killing tumors. Further, it is more effective when combined with a chemotherapeutic agent.