摘要
建立反相离子对HPLC法测定复方苯海拉明搽剂中二组分的含量。采用Diamonsil C18为色谱柱,以0.15%庚烷磺酸钠溶液(用磷酸调节pH值为3.5)-甲醇(38:62)为流动相,检测波长为220nm,流速为1.0mL.min-1。盐酸苯海拉明及苯佐卡因的线性范围分别为1.6～40.5μg.mL-1(r=0.9992)及3.3～81.5μg.mL-1(r=0.9991),平均回收率分别为99.6%(RSD=0.86%)及99.7%(RSD=1.1%)。本方法简单、迅速、准确,可用于复方苯海拉明搽剂中二组分的含量测定。
To establish a RP- Ion pair HPLC method for assay of two composition in Compound Diphenhydramine Hydrochloride Liniment . The method was carried out on the diamonsil C18 Column, column temperature. 30℃, 0. 15% sodium heptanesulfonate （adjust pH value to 3. 5 with phosphoric acid） -methanol （38 ： 62） was used as the mobile phase , the detection wavelength was 220nm, flow rate ： 1.0mL·min^-1. The linear ranges for diphenhydramine hydrochloride and benzocaine were 1.6 40. 5μg·mL^-1 （r=0. 9992） and 3.3-81.5μg ·mL^-1 （r=0. 9991）, respectively, and the average recoveries rates of method were 99.6% （RSD=0. 86%） and 99.7% （RSD= 1.1%） respectively. The method is simple, quick and accurate, it can be used for quality control of two composition in the drug.
出处
《中国药事》
CAS
2007年第10期821-823,共3页
Chinese Pharmaceutical Affairs
