RP-HPLC法测定浓缩六味地黄丸中马钱苷的含量

Determination of Loganin in Enriched Liuweidihuang Pills by RP-HPLC

建立浓缩六味地黄丸中马钱苷的含量测定方法。采用高效液相色谱法,以Hypersil ODS2为色谱柱;流动相:0.05mol.L-1磷酸二氢钠-乙腈(85:15);检测波长:238nm。马钱苷在0.0285～5.2250μg范围内,浓度与峰面积呈良好的线性关系(r=0.9998);回收率:98.39%,RSD=1.2%(n=9)。此方法简便、快速,可作为浓缩六味地黄丸中马钱苷的含量测定方法。

To investigate a method for determination of loganin in enriched Liuweidihuang Pills. Hypersil ODS2 column was used 0.05mol·L^-1 NaH2PO4-acetonitrile （85 ： 15） was used as mobile phase. The photodiode array detector was used at 238nm. The calibration curve was linear over the range of 0. 0285-5. 2250vg （r=0. 9998） . The average recovery of the method was 98. 39%, RSD=1. 2% （n =9） . This method is reliable and accurate. The method can be applied to separate and determine the loganin in enriched Liuweidihuang Pills.