癌基因C-MYC与结肠癌染色体不稳定的研究

Deregulation of C-MYC induces chromosomal instability in human colon cancer

目的探讨癌基因C-MYC的表达对人类结肠癌细胞系DLD1染色体稳定性的影响。方法建立四环素调控的C-MYC稳定表达的细胞系DLD1tTA-C-MYC,Western blot检测C-MYC的稳定表达。C-MYC持续稳定表达1、5、7和14d后,对细胞进行DAPI染色,在荧光显微镜下计数染色体不稳定细胞的百分比。结果Western blot显示C-MYC的表达在72h后达最高峰。C-MYC表达关闭的DLD1细胞中,染色体不稳定细胞的百分比为1.51%;而C-MYC持续表达1、5、7和14d后的DLD1细胞中,染色体不稳定细胞的百分比分别为2.2%、3.6%、4.7%和6.13%(P<0.01)。结论C-MYC的高表达可导致DLD1结肠癌细胞非整倍体数染色体的形成,也是造成结肠癌染色体不稳定的重要因素。

To study the influence of stably inducible expression of C-MYC on the chromosomal instability in human colon cancer cell line DLD1. Methods Tetracycline-responsive geneinducible cell line DLDltTA-C-MYC was generated. Western blot was used to examine the induction of C-MYC expression upon removal of doxycycline. 4-6-Diamidino-2-phenylindole（DAPI）staining was performed to detect the percentage of cells with chromosomal instability 1,5,7 and 14 days after the induction of C-MYC expression. Results Western blot showed that the peak of C-MYC expression appeared 72h after induction. The percentage of cells with chromosomal instability in tet-off DLDltTA-C- MYC cells was 1.51%. However, the percentages of cells with chromosomal instability were 2. 2%, 3.6%,4. 7% and 6.13% respectively, 1,5,7 and 14 days after the induction of C-MYC express ion（P %0. 01 ）. Conclusion Deregulation of C-MYC can induce the formation of aneuploidy in human colon cancer cell line DLD1, and it also plays an important role in the pathway of chromosomal instability.