醒脑开窍针刺法对不同时间局灶性脑缺血/再灌注损伤时活体脑片海马神经元[Ca^(2+)]_i的影响

Effects of "XNKQ" acupuncture on [Ca^(2+)]i after different lengths of cerebral ischemia reperfusion(CIR) in rat hippocampus

目的:研究醒脑开窍针刺法对局灶性脑缺血/再灌注大鼠脑细胞内Ca2+的调节作用。方法:利用激光共聚焦扫描显微镜观察海马CA1区活体脑片中锥体细胞内Ca2+的分布及动态变化。结果:脑缺血1h时海马CA1区神经元的[Ca2+]i明显升高,再灌注后,神经元内[Ca2+]i随再灌注时间延长而进一步升高,到再灌注6h,达到最高峰,此后虽有下降,但仍高于缺血前水平,再灌注24h时又有第二次钙积聚高峰。针刺对脑缺血/再灌注大鼠各时程的[Ca2+]i均有降低作用,但对其下调程度,醒脑开窍针刺组明显优于传统针刺组。结论:醒脑开窍针刺法可迅速调节缺血/再灌注后海马CA1区神经元细胞内的Ca2+含量,抑制胞内钙超载,拮抗脑缺血/再灌注后继发神经元的损伤。

Objective: To study the 'XNKQ' acupuncture's intervention influence on hippocampus CA1 neuron [Ca2+]i during CIR.Methods:Using confocal laser scanning microscope(CLSM) to observe the distribution and dynamic changes of the intracellular free calcium in hippocampus CA1 neuron.Results:The neuron [Ca2+]i of hippocampus CA1 regions in the rat began to increase after ischemia 1h, and sustained to elevate after reperfusion, at 6h of ischemia reperfusion time course the [Ca2+]i elevated at the highest level. Then the [Ca2+]i became to decrease, however is still higher than that before CIR. While at 24h the [Ca2+]i elevated at the second peak of calcium accumulation, but is lower than 6h time course. Both 'XNKQ' 1acupuncture and traditional acupuncture could decrease the ischemic accumulation of calcium of any time course during CIR. But the inhibitory effect to Ca2+ overloading of 'XNKQ' acupuncture is obviously better than that of traditional acupuncture.Conclusion:The 'XNKQ' acupuncture could regulate the content of intracellular calcium of the region of ischemia and reperfusion, inhibited Ca2+ overloading, and maintained the calcium homeostasis, so as to protect neurons from injury of brain ischemia reperfusion.