白细胞介素-1β对人冠脉平滑肌细胞基质金属蛋白酶系统分泌平衡的影响

The Influence of Interleukin-1β on Matrix Metalloproteinases System Protein Expression Balance of Human Cornary Artery Smooth Muscle Cells

目的：研究白细胞介素-1β（Interleukin-1β,IL-1β）对人冠状动脉平滑肌细胞（human coronary artery smooth muscle cell,HCASMC）基质金属蛋白酶3（matrix metalloproteinase3,MMP-3）金属蛋白酶组织抑制因子1（tissue inhibitor of metallopro-teinase1,TI MP-1）分泌平衡的影响。方法：培养HCASMC,至第5-7代时,分别加入20μg·L^-1和0μg·L^-1IL-1β（0μg·L^-1组设为对照组）,均分别孵育2h、4h、8h、24h、36h后,收集细胞培养液上清;采用IL-1β（0、5、20、40μg·L^-1）刺激HCASMC6h后分别收集细胞和细胞培养液上清。采用ELISA法检测细胞培养液上清内MMP-3及TI MP-1的表达量。结果：IL-1β组MMP-3,TI MP-1的表达量在2h时开始增加,并随时间的延长而不断增加,MMP-3/TI MP-1在2h开始升高,8h达到顶峰,后缓慢下降;随IL-1β剂量增加,MMP-3和TI MP-1的表达量、MMP-3/TI MP-1均不断升高。结论：IL-1β可造成HCASMC分泌不稳定斑块标记物MMP-3、TI MP-1及MMP-3/TI MP-1的升高,说明炎症可能是急性冠状动脉综合征发生发展的机制之一。

Objective. To observe the effect of IL-1β on secretions balance of MMP-3 and TIMP-1 in HCASMC. Medthods： Incubation of HCASMC with IL-1β （20μg·L^-1and 0μg·L^-1）, respectively,collect culture media after 2 h, 4 h, 8 h, 24 h and 36 h;stimulate HCASMC with IL-1β（0, 5, 20, 40μg·L^-1 ）, respectively, collect culture media after 6 h. Measure the concentrations of MMP-3 and TIMP-1 in cultural fluid by ELI.ISA. Results.. Incubation of HCASMC with 20μg·L^-1 IL-1β results in a time-dependent increase of MMP-3 and TIMP-1＇s secreations, and MMP-3/TIMP-1 is ul〉regulation at 2 hour,get to the peak at 8 hour, then begin to descent slowly. Incubation the HCASMC with different concentrations of IL-1β caused a dose-dependent increase in MMP-3 and TIMP-1＇s secreations and a increase of MMP-3/TIMP-1. Conehlsion ： IL-1β can induces the HCASMAC＇s production of MMP-3 and TIMP-1 ,also the MMP-3/TIMP-1 ,and thus has implicated that inflammation may paly an important role in the development of acute coronary syndroms.