益生菌VSL# 3对Oxazolone小鼠结肠炎结肠黏膜中β防御素-2表达的影响

Effects of probiotic bacteria VSL# 3 on the expression of β defensin 2 in Oxazolone induced murine colitis

目的观察益生菌VSL#3对Oxazolone小鼠结肠炎结肠黏膜中β防御素-2表达的影响。方法建立Oxazolone小鼠结肠炎模型并分为VSL#3治疗组、SASP治疗组、治疗对照组。免疫组化方法、RT-PCR及Western blot检测各组结肠粘膜组织中β防御素-2的蛋白及mRNA的表达。结果免疫组化方法、RT-PCR检测及Western blot检测发现VSL#3治疗组小鼠结肠粘膜中β防御素-2的蛋白及mRNA的表达低于治疗对照组,与SASP治疗组比较差异没有显著性。结论VSL#3对Oxazolone诱导的小鼠结肠炎有治疗作用,其作用机制可能与下调β防御素-2的表达有关。

Objective To investigate the effects of probiotic bacteria VSL#3 on the expression of βdefensin 2 in colonic mucosa of mice with oxazolone-induced murine colitis. Methods The colitis model was established. The healthy female Balb/c mice of 6 to 8 weeks were divided into three groups ： VSL # 3 treatment group, SASP treatment group and control group. The expression ofβdefensin 2 in colonic mucosa were studied by immunohistochemistry assay, RT-PCR and Western blot analysis in three groups. Results The expression of β defensin 2 in colonic mucosa in VSL 3 treatment group was significantly lower than that in control group （P〈0.05）; there was no significant differences between VSL # 3 group and SASP group （P〉0.05）. The data measured by RT-PCR showed the expression of β defensin-2 mRNA in VSL # 3 treatment group was significantly lower than that in control group （P〈0.05）. There were no significant differences in VSL#3 and SASP groups （P〉0.05）. The expression of βdefensin 2 proteins in VSL # 3 treatment group by Western blot analysis was significantly lower than that in control group （P〈0.05）. There was no significant difference between VSL # 3 and SASP groups for prevention and treatment. Conclusion VSL # 3 is effective in the treatment of oxazolone induced colitis, which may be correlated to down regulation of the expression of βdefensin 2.