血管紧张素原T174M基因多态性与原发性高血压的关系

Relationship between angiotensinogen gene T174M polymorphism and essential hypertension

目的研究血管紧张素原T174M基因多态性与原发性高血压的关系。方法采用聚合酶链反应、限制性内切酶酶解(PCR-RFLP)及电泳分型的方法对四川泸州及其附近地区140例原发性高血压患者和40例血压正常者血管紧张素原的T174M基因多态性进行检测。结果高血压组与正常对照组比较,基因型频率分布差异无显著性,高血压组TT型、MT型和MM型分别为63.6%、33.6%、2.8%,对照组分别为72.5%、27.5%、0%;等位基因频率两组不同,高血压组M等位基因频率为19.6%,对照组为13.8%。高血压组T等位基因频率为80.4%,对照组为86.3%。结论血管紧张素原T174M基因多态性可能与四川川南地区人群原发性高血压有关。

Objective To investigate the relationship between T174Mallele polymorphism in exon 2 of angiotensinogen （AGT） gene and essential hypertension （EH）. Methods Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene polymorphism in 40 normotensive controls and 140 primary hypertensive patients. Results The frequencies of TT, MT, MM genotype were 63.6% ,33.6% ,2.8% in hypertensive patients and 72. 5%, 27.5%, 0% in normotensive controls, respectively. The frequencies of T allele and M allele were 80.4%, 19. 6% in hypertensive patients and 86.3%, 13. 8% in normotensive controls, respectively. No statistically significant differences were found between two groups （ P 〉0.05）. Conclusion The T174M variation of AGT gene may be associated with the essential hypertension in South Sichuan.