摘要
报道高脚小伞菌丝体原生质体制备及再生的结果．多种因素影响原生质体的产量及再生率，在最适条件下，原生质体产量可达２．５４×１０６个／ｍｌ，再生率达２３．６％，还对原生质体的活力及核数进行了测定。
Influence of factors on the protoplast separation and regeneration of mycelia from M.procera have been studied. In best conditions, the protoplast yield was up to 2.54×10 6 /ml and the regeneration ratio was 23.6% .The activity of protoplast was affected by factors of time and temperature of incubation, pH ect. The activity of protoplast and the number unclei in protoplasts are concerned with the regeneration ratio of protoplast.
出处
《湖南师范大学自然科学学报》
CAS
1997年第2期82-86,共5页
Journal of Natural Science of Hunan Normal University
基金
国家科委重点课题
湖南省重点学科资助
关键词
高脚小伞
原生质体
制备
再生
丝状真菌
Macrolepiota procera Sing
separation
regeneration
protoplast
