藜芦的黄酮类化学成分研究

Studies on the chemical constituents of flavones of Veratrum nigrum L.

目的：对百合科藜芦属植物藜芦Veratrum nigrumL.的黄酮类化学成分进行分离和结构鉴定。方法：采用溶剂法、硅胶柱层析、Sephadex LH-20柱层析进行成分分离和纯化,通过理化性质和波谱技术鉴定化合物的结构。结果：从藜芦乙醇提取物中分离鉴定了9个黄酮类化合物,分别鉴定为3-甲氧基异鼠李素（3-methoxylisorhamnetin,1）、异鼠李素（isorham-netin,2）、3-甲氧基槲皮素（3-methoxylquercetin,3）、槲皮素（quercetin,4）、异槲皮苷（isoquercitrin,5）、3,5,7-三羟基-3′,5′-二甲氧基黄酮（3,5,7-trihydroxy-3′,5-′dimethoxylflavone,6）、5,7,4′-三羟基-3′-甲氧基黄酮（5,7,4-′trihydroxy-3-′methoxyl-fla-vone,7）、5,7,3′,4′-四羟基黄酮（5,7,3′,4-t′etrahydroxyflavone,8）、5,7,3′,5′-四羟基黄酮（5,7,3′,5-′tetrahydroxyflavone,9）。结论：化合物1-9均为首次从该植物中分离得到。

Objective：To investigate the chemical constituents of flavones of Veratrum nigrum L..Methods：The compounds were isolated by repeated column chromatography over Sephadex LH-20,RP C18 and silica gel.The compounds obtained were identified according to their physicochemical properties and spectral data（UV,ESI-MS,1H-NMR and13C-NMR）.Results：Nine compounds were isolated and identified as 3-methoxylisorhamnetin（1）,isorhamnetin（2）,3-methoxylquercetin（3）,quercetin（4）,isoquercitrin（5）,3,5,7-trihydroxy-3′,5′-dimethoxylflavone（6）,5,7,4′-trihydroxy-3′-methoxylflavone（7）,5,7,3′,4′-tetrahydroxyflavone（8）,5,7,3′,5′-tetrahydroxyflavone（9）,respectively.Conclusion：All of the compounds 1-9 are separated from Veratrum nigrum L.for the first time.