橄榄总黄酮对大鼠局灶性脑缺血/再灌注损伤的保护作用

Protective effect of total flavanone from Chinese Olive on cerebral ischemia-reperfusion injury in rats

目的观察橄榄总黄酮(total flavaone fromChinese Olive)对大鼠局灶性脑缺血/再灌注损伤的保护作用。方法线拴法阻塞大鼠中动脉2h/再灌注24h,观察橄榄总黄酮30、60mg.kg-1对神经功能缺失、脑梗塞体积、脑组织髓过氧化物酶(MPO)、还原型谷胱甘肽(GSH)、总抗氧化能力(T-AOC)的影响。结果橄榄总黄酮30、60mg.kg-1均对大鼠神经功能障碍有改善作用,明显缩小脑梗死灶,增加GSH活力和总抗氧化能力(T-AOC),缓解MPO升高。结论橄榄总黄酮对大鼠局灶性脑缺血/再灌注损伤有保护作用。

OBJECTIVE To investigate the protective effect of total flavanone from chinese olive on focal cerebral ischemia - reperfusion injury in rats. METHODS The right middle cerebral artery of the rat was occluded by inserting a thread through internal carotid artery for 2h, and then reperfused for 24h. total flavanone from Chinese oli- It ve（30,60mg · kg^-1 ）was injected for 4d before ischemia. After reperfusion 24h, the extent of neurological deficits was evaluated by Longer＇s method ; the infarct size of the brain were measured by AutoCAD image analysis soft ware; the morphological changes of the brain tissue were observed; MPO,GSH and T - AOC level in the brain tissue were detected. RESULTS Compare with vehicle group ,after 2h of occlusion and 24h of reperfusion, the neurological score were evidently reduced ;the brain infarct size were significantly reduced in 30 and 60 mg· kg^-1 total flavanone of Chinese olive groups ; total flavanone of Chinese olive also increased the level of GSH and T - AOC , and inhibited the level of MPO in brain tissue. CONCLUSION Total flavanone of Chinese olive possesses the neuroprotective effects on focal cerebral ischemia -reperfusion injury in rats.