HPLC-ELSD法测定复方氨酚葡锌片中葡萄糖酸锌和对乙酰氨基酚的含量

Determination of zinc gluconate and paracetamol in compound paracetamol and zinc gluconate tablets by HPLC-ELSD

目的建立一种快速，准确的HPLC-ELSD法测定复方氨酚葡锌片中葡萄糖酸锌和对乙酰氨基酚含量的方法。方法HPLC-ELSD法，色谱柱：Kromasil C18（4．6mm×250mm，5μm）；流动相：乙腈-水（20：80）；检测温度：41℃；流速：1．0mL·min^-1；进样量为201J,L；雾化气体N2流速2．5L·min^-1。结果葡萄糖酸锌和对乙酰氨基酚分别在0．09976—0．1796mg·mL^-1、0．1385～0．2493mg·mL^-1范固内浓度与峰面积具有良好的线形关系（r分别为0．9985，0．9992）；葡萄糖酸锌和对乙酰氨基酚的平均回收率分别为98．78％和98．70％，RSD分别为0．62％和0．36％（n=9）。结论本法快速，简便，灵敏度高，重现性好。

OBJECTIVE To develop a rapid and accurate method for determining the contents of Zinc gluconate and paracetamol in compound paracetamol and Zinc gluconate tablets by HPLC-ELSD. METHODS HPLC-ELSD, the analytical column was Kromasil C18 （4. 6mm × 250mm ,5μm） ;The mobile phase was acetonitrile： water（40： 60） ; the detector temperature was 41℃ ;the flow rate was 1.0mL·mL^-1. RESULTS The logarithm of the contents of Zinc gluconate had good linear relationship with the logarithm of their peak area in the scope of 0. 09976 -0. 1796mg ·mL^-1 （r = 0. 9985 ）, and the logarithm of the contents of paracetamol had good linear relationship with the logarithm of their peak area in the scope of 0. 1385 -0. 2493 mg ·mL^-1（ r = 0. 9992）. The average recoveries were 98.78%（RSD =0.36%） and 98.70% （RSD =0.62%） （n =9）. CONCLUSION The results showed that the method was rapid, simple, reliable, and had excellent precision.