炭载肌红蛋白作生物燃料电池阴极催化剂

Myoglobin Adsorbed on Active Carbon as Cathodic Catalyst in Biofuel Cell

采用平衡吸附法制备了吸附在活性炭上的肌红蛋白(Mb-C)催化剂。循环伏安测量表明,吸附Mb的氧化还原峰分别位于-342和-402mV,氧化还原峰峰电流基本相等,峰电流与扫描速率成正比关系。说明Mb已经吸附到活性炭上,吸附的Mb能进行非扩散的、直接的准可逆电化学反应;用Nafian膜把Mb-C固定在在玻碳(GC)电极上后的稳定性很好,浸泡7d后的Mb-C/GC电极的循环伏安曲线与新制备的基本相同,电极上Mb的表观吸附量为4.5×10-10mol/cm2。Mb-C催化剂对O2的电化学还原具有很好的催化活性,说明吸附在活性炭上的Mb能保持其生物活性。Mb-C催化剂可用作生物燃料电池的阴极催化剂。

Active carbon supported myoglobin（Mb-C） catalyst was prepared via the balance adsorption method. The cyclic voltammetric measurements indicated that the redox peaks of Mb adsorbed on the active carbon were located at - 342 and - 402 mV, respectively. The currents of the redox peaks are basically equal. The peak currents were proportional to the scan rate. These results indicated that Mb adsorbed on the active carbon and underwent a non-diffused, direct and quasi-reversible electrochemical reaction. The stability of the Mb-C catalyst fixed on the glassy carbon （GC） electrode by Nation membrane was excellent. The cyclic voltammogram of the Mb-C/GC electrode immersed in the phosphate buffer solution for 7 days was similar to that measured immediately after preparation. The amount of Mb adsorbed on the electrode was calculated to be 4. 5 × 10^-10 mol/cm^2. In addition, the Mb-C catalyst showed a good electrocatalytic activity for the reduction of O2, illustrating that the Mb adsorbed on the active carbon was not denatured and thus its bioactivity was maintained. Thus, the Mb-C catalyst can be used as the cathodic catalyst in the biofuel cell.