摘要
目的:研究以生物可降解壳聚糖纳米粒作为粒细胞-巨噬细胞集落刺激因子(GM-CSF)新型缓释系统的可行性。方法:以三聚磷酸钠为交联剂,采用离子交联法制备负载GM-CSF和牛血清白蛋白(BSA)的纳米粒。用透射电镜观测纳米粒径和形态;用紫外分光光度计、荧光分光光度计分别测定BSA和GM-CSF包封率,并考察制剂体外药物释放情况。结果:纳米粒形态多呈球形,平均粒径为201nm,GM-CSF和BSA包封率分别为62.1%、58.5% ,3d时体外累积释放率分别为69%、82%。结论:应用离子交联法可制备负载GM-CSF的壳聚糖缓释纳米粒。
OBJECTIVE: To study the feasibility of using biodegradable chitosan nanoparticles as new type carriers for control0led GM - CSF delivery system. METHODS: GM - CSF and BSA loaded chitosan nanoparticles were prepared by ion cross linking method using tripolyphosphate as cross- linking agent with its morphology observed by transmission electron microscope. The encapsulating efficiencies of GM - CSF and BSA were determined respectively by fluorescence scanning and ultraviolet spectrophotometer. The in vitro drug release properties of chitosan nanoparticles were studied as well. RESULTS: Morphologically, the nanoparticles were spherical with mean particle size of 201nm. The encapsulating efficiencies of GM- CSF and BSA were 62.1% and 58.5%, respectively, and their in vitro accumulative drug release rates on the 3rd day were 69% and 82% respectively. CONCLUSION: The ion cross linking method is fit for the preparation of GM- CSF loaded chitosan nanoparticles.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第31期2435-2437,共3页
China Pharmacy