粒细胞-巨噬细胞集落刺激因子壳聚糖缓释纳米粒的制备

Preparation of GM-CSF Loaded Chitosan Nanoparticles

目的:研究以生物可降解壳聚糖纳米粒作为粒细胞-巨噬细胞集落刺激因子(GM-CSF)新型缓释系统的可行性。方法:以三聚磷酸钠为交联剂,采用离子交联法制备负载GM-CSF和牛血清白蛋白(BSA)的纳米粒。用透射电镜观测纳米粒径和形态;用紫外分光光度计、荧光分光光度计分别测定BSA和GM-CSF包封率,并考察制剂体外药物释放情况。结果:纳米粒形态多呈球形,平均粒径为201nm,GM-CSF和BSA包封率分别为62.1%、58.5% ,3d时体外累积释放率分别为69%、82%。结论:应用离子交联法可制备负载GM-CSF的壳聚糖缓释纳米粒。

OBJECTIVE： To study the feasibility of using biodegradable chitosan nanoparticles as new type carriers for control0led GM - CSF delivery system. METHODS： GM - CSF and BSA loaded chitosan nanoparticles were prepared by ion cross linking method using tripolyphosphate as cross- linking agent with its morphology observed by transmission electron microscope. The encapsulating efficiencies of GM - CSF and BSA were determined respectively by fluorescence scanning and ultraviolet spectrophotometer. The in vitro drug release properties of chitosan nanoparticles were studied as well. RESULTS： Morphologically, the nanoparticles were spherical with mean particle size of 201nm. The encapsulating efficiencies of GM- CSF and BSA were 62.1% and 58.5%, respectively, and their in vitro accumulative drug release rates on the 3rd day were 69% and 82% respectively. CONCLUSION： The ion cross linking method is fit for the preparation of GM- CSF loaded chitosan nanoparticles.