低密度脂蛋白对体外培养的肾小球系膜细胞表型转化的影响

The Effect of Low Clensity Lipoprotein on Phenotype Change of the Cultured Rat Glomerular Mesangial Cells

目的通过低密度脂蛋白(LDL)的刺激,观察LDL对体外培养的大鼠肾小球系膜细胞表型转化的诱导作用。方法采用不同浓度的LDL(0,25,50,100,150μg/ml)刺激体外培养的大鼠肾小球系膜细胞的水平,以台盼蓝染色检测LDL对系膜细胞的毒性作用,MTT比色法检测细胞增殖,透射电镜观察系膜细胞的形态学变化,免疫细胞化学染色及Western blot检测α-SMA、CTGF、Ⅳ型胶原及FN的表达。结果在LDL作用下,倒置显微镜下细胞变为长梭形,透射电镜下细胞表面微绒毛减少。免疫细胞化学染色及Western blot显示α-SMA、CTGF、Ⅳ型胶原及FN表达增强。结论在LDL刺激下,系膜细胞可以发生表型转化。

Objective To study the influence of low density lipoprotein on phenotype change of the cultured rat glomerular mesangial cells（MCs）. Methods The rat glomerular mesangial cells were cultured for seven days in low density lipoprotein（0,25,50,100,150μg/ml）, MCs proliferation was measured under MTT assay, morphological changes were observed by electronic microcopy, α- SMA, CTGF,ColⅣand FN were examined by immunohistochemistry stain and Western blot. Results Glomerular mesangial cells cultured in low density lipoprotein showed obvious morphlogic changes, including elongated shape and the appearance of muscle actin. The expression amount of α- SMA, CTGF,ColⅣ and FN enhanced by using immunohistochemistry stain and Western blot. Conclusion Low density lipoprotein can lead to the phenotypic change of glomerular mesangial cells.