富铁酵母的选育研究

Research on screening of iron-enriched yeasts

通过对10株酿酒酵母菌株进行初筛、复筛、驯化得到1株富铁能力强的9F菌株。对9F菌株的发酵条件进行优化得到:10°Bx的麦芽汁,8g/L尿素,0.02g/L磷酸二氢钾,1200g/mLFe2+,培养基初始pH值自然,装液量50mL/250mL,32℃发酵24h,生物量达11.08g/L,铁含量达32.56mg/g酵母,富集率达到30.10%,同时得到该菌株生长规律和动力学关系式。

The iron-enriched yeast strain 9F was obtained by primary screening, second screening and domesticating of 10 different Saccharomyces cerevisiae. The fermentation conditions of strain 9F was optimized as follows： 10°Bx wort, 8 g/L urea, 0.02 g/L KH2PO4, 1200 g/ml Fe^2＋, initial pH was natural, liquid medium volume was 50 ml/250 ml, fermentation temperature was 32℃ and fermentation time was 24 h. Under above conditions, the biomass, iron content and concentration rate of 9F reached 11.08 g/L, 32.56 mg/g and 30.10% , respectively. The growth pattern of 9F was investigated to reveal a kinetic relationship.