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山羊痘病毒结构蛋白P32基因的表达 被引量：2

Prokaryotic expression of structural protein P32 gene of goat pox virus
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摘要 将山羊痘病毒结构蛋白P32基因从重组质粒pMD-P32中亚克隆至pGEX-4T-1原核表达载体上,用构建的重组表达质粒pGEX-P32转化大肠杆菌BL21(DE3)感受态细胞,1 mmol/L IPTG诱导表达,细菌裂解物经SDS-PAGE检测到分子量约为58 ku的目的蛋白,与预期的产物大小一致.Western-blotting表明该重组蛋白可被山羊痘阳性血清所识别. The structural protein P32 gene of the goat pox virus in the recombinant plasmid pMD-P32 was subcloned into a prokaryotic expression vector pGEX-4T-1, and the recombinant plasmid was trans- formed into E. coli BL21（DE3）. The recombinant fusion protein about 58 ku was expressed in E, coli induced by 1 mmol/L IPTG,and western-blotting test showed that the protein could be recognized by antiserum against goat pox virus.
作者 张强 马维民 吴国华 颜新敏 李健 朱海霞 朱彩珠 吴润 刘湘涛 才学鹏
机构地区 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室 甘肃农业大学动物医学院
出处 《甘肃农业大学学报》 CAS CSCD 2007年第5期5-8,共4页 Journal of Gansu Agricultural University
基金 国家基础平台项目(2005DKA21205-3)资助
关键词 羊痘病毒 P32基因 原核表达 goat pox virus P32 gene prokaryotic expression
分类号 S852.654 [农业科学—基础兽医学] Q786 [生物学—分子生物学]
  • 相关文献

参考文献10

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二级参考文献12

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共引文献18

同被引文献11

  • 1王开功,虞天德,何光志,周碧君,许乐仁,孙道忠.应用反向间接血凝试验检测山羊痘病毒抗原[J].山地农业生物学报,2005,24(1):29-32. 被引量：21
  • 2马维民,刘湘涛,张强,吴国华,颜新敏,李健,朱海霞,吴润.山羊痘病毒P32基因的克隆与序列分析[J].甘肃农业大学学报,2006,41(4):27-30. 被引量：19
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  • 9周碧君,岳筠,徐春志,程振涛.山羊痘病毒的分离与鉴定[J].中国预防兽医学报,2007,29(9):661-664. 被引量：8
  • 10芦晓立,颜新敏,吴国华,叶奕优,李健,朱海霞,王建科,赵志荀,崔力凡,朱彩珠,张强.绵羊CD58基因的克隆与原核表达[J].甘肃农业大学学报,2010,45(5):11-14. 被引量：4

引证文献2

二级引证文献9

甘肃农业大学学报
2007年 第5期

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