摘要
将山羊痘病毒结构蛋白P32基因从重组质粒pMD-P32中亚克隆至pGEX-4T-1原核表达载体上,用构建的重组表达质粒pGEX-P32转化大肠杆菌BL21(DE3)感受态细胞,1 mmol/L IPTG诱导表达,细菌裂解物经SDS-PAGE检测到分子量约为58 ku的目的蛋白,与预期的产物大小一致.Western-blotting表明该重组蛋白可被山羊痘阳性血清所识别.
The structural protein P32 gene of the goat pox virus in the recombinant plasmid pMD-P32 was subcloned into a prokaryotic expression vector pGEX-4T-1, and the recombinant plasmid was trans- formed into E. coli BL21(DE3). The recombinant fusion protein about 58 ku was expressed in E, coli induced by 1 mmol/L IPTG,and western-blotting test showed that the protein could be recognized by antiserum against goat pox virus.
出处
《甘肃农业大学学报》
CAS
CSCD
2007年第5期5-8,共4页
Journal of Gansu Agricultural University
基金
国家基础平台项目(2005DKA21205-3)资助
关键词
羊痘病毒
P32基因
原核表达
goat pox virus
P32 gene
prokaryotic expression