二硫苏糖醇处理淋巴细胞毒高敏感肾移植透析患者抗体的研究

Dithothreitol (DTT) treating lymphocytes highly sensitized antibodies in renal pretransplantation patients

本文用35名无关供者淋巴细胞对72份淋巴细胞抗体高敏感患者血清进行了抗体检测，同时用二硫苏糖醇（DTT）处理了血清。结果表明，72份血清在4℃和37℃均对B淋巴细胞反应，而对T淋巴细胞则较少反应。肾移植术后，由于排斥而切除移植肾的患者抗T、B淋巴细胞抗体高于输血和妊娠患者，而输血所致的淋巴细胞抗体次之。DTT处理血清后证明，高敏感患者的抗体一般是IgG和IgM混合型，抗体产生的机率仍是IgG高。

72 sera of highly sensitized antibody patients were detected by 35 samples of undonor lymphocytes be fore and after the dithothreitol (DTT) treating. The results showed that 72 sera reacted completely to B lymphocytes in both 4℃ and 37℃， but incompletely to T lymphocytes. After removal of the transplant kidney due to rejection, the sera antibody levels of T or B lymphocytes were higher than those of the patients. sera after blood transfusion or pregnancy.The serum T or B antibody levels of pregnancy were the lowest. ln DTT-treated sera of highlysensitized patients, the antibodies were mixed types of IgG and IgM.