摘要
目的:观察MMP-2、MMP-9、VEGF和RECK基因在膀胱癌组织中的表达,探讨膀胱癌转移可能的基因机制。方法:采用RT-PCR法检测50例膀胱癌手术切除标本中MMP-2、MMP-9、VEGF和RECK基因的表达,并分析其临床意义。结果:MMP-2、MMP-9、VEGF和RECK在膀胱癌组织中的阳性表达率分别为80%、56%、78%和64%。膀胱癌组织MMP-2、MMP-9和VEGF三种基因的表达水平高于癌周组织(P<0.05),而RECK基因的表达水平低于癌周组织(P<0.05)。膀胱癌组织中MMP-2、MMP-9和VEGF基因的表达水平与膀胱癌组织的病理分级分期密切相关,而RECK基因的表达水平与膀胱癌的分级分期无明显相关。结论:膀胱癌组织存在多基因的表达异常,其发生、发展及转移与多基因联合作用有关。
Objective:To study the expression of MMP 2, MMP-9, vascular endothelial growth factor (VEGF) and RECK genes in bladder cancer tissues, so as to provide evidence for studying the molecular mechanism of bladder cancer metastasis. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to determine the expression of MMP-2, MMP- 9, VEGF and RECK genes in 50 operative specimens of bladder cancer and their corresponding adjacent normal tissues; and the clinical significance of the expression was analyzed. Results: The positive rates of MMP-2, MMP-9, VEGF and RECK mRNA in the bladder cancer specimens were 80%, 56%, 78% and 64%, respectively. The expression levels of MMP-2, MMP-9 and VEGF mRNA in bladder cancer specimens were significantly higher than those in the paired adjacent normal bladder tissues(P〈0.05) ; the expression level of RECK genes in bladder cancer specimens was significantly lower than that in the normal tissue (P〈0.05). The expression levels of MMP-2, MMP-9 and VEGF was closely correlated with the clinical stages and grades of bladder cancer; there was no relation between the level of RECK expression and clinical stages and grades of the cancer. Conclu- sion: Abnormal expression of multi genes is present in bladder cancer tissues; the development, progression and metastasis of bladder cancer are associated with abnormal expression of multi-genes.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第10期1075-1078,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30270522)
上海市科委基金(054119604).~~
关键词
膀胱肿瘤
多基因表达
肿瘤转移
bladder neoplasms
expressions of multiple gene
neoplasm metastasis