膀胱癌组织中MMP-2、MMP-9、VEGF和RECK基因的表达及临床意义

Expression of MMP-2,MMP-9,VEGF and RECK genes in bladder cancer and its clinical significance

目的:观察MMP-2、MMP-9、VEGF和RECK基因在膀胱癌组织中的表达,探讨膀胱癌转移可能的基因机制。方法:采用RT-PCR法检测50例膀胱癌手术切除标本中MMP-2、MMP-9、VEGF和RECK基因的表达,并分析其临床意义。结果:MMP-2、MMP-9、VEGF和RECK在膀胱癌组织中的阳性表达率分别为80%、56%、78%和64%。膀胱癌组织MMP-2、MMP-9和VEGF三种基因的表达水平高于癌周组织(P<0.05),而RECK基因的表达水平低于癌周组织(P<0.05)。膀胱癌组织中MMP-2、MMP-9和VEGF基因的表达水平与膀胱癌组织的病理分级分期密切相关,而RECK基因的表达水平与膀胱癌的分级分期无明显相关。结论:膀胱癌组织存在多基因的表达异常,其发生、发展及转移与多基因联合作用有关。

Objective：To study the expression of MMP 2, MMP-9, vascular endothelial growth factor （VEGF） and RECK genes in bladder cancer tissues, so as to provide evidence for studying the molecular mechanism of bladder cancer metastasis. Methods： Reverse transcription polymerase chain reaction （RT-PCR） was used to determine the expression of MMP-2, MMP- 9, VEGF and RECK genes in 50 operative specimens of bladder cancer and their corresponding adjacent normal tissues; and the clinical significance of the expression was analyzed. Results： The positive rates of MMP-2, MMP-9, VEGF and RECK mRNA in the bladder cancer specimens were 80%, 56%, 78% and 64%, respectively. The expression levels of MMP-2, MMP-9 and VEGF mRNA in bladder cancer specimens were significantly higher than those in the paired adjacent normal bladder tissues（P〈0.05） ; the expression level of RECK genes in bladder cancer specimens was significantly lower than that in the normal tissue （P〈0.05）. The expression levels of MMP-2, MMP-9 and VEGF was closely correlated with the clinical stages and grades of bladder cancer; there was no relation between the level of RECK expression and clinical stages and grades of the cancer. Conclu- sion： Abnormal expression of multi genes is present in bladder cancer tissues; the development, progression and metastasis of bladder cancer are associated with abnormal expression of multi-genes.