组合抗原ELISA法和潜伏态IFA在HHV-8血清检测中的对比研究

Two serum assay for human hepersvirus 8:combined-antigen-ELISA assay and the latency-IFA assay

目的比较HHV-8组合抗原ELISA检测法与潜伏态病毒IFA法的检测灵敏性与特异性,并验证组合抗原ELISA法作为分子流行病学调查方法的可行性。方法组合抗原ELISA检测法与潜伏态病毒IFA法对32例法国AIDS相关KS患者血清和23例法国肝移植后皮肤癌患者血清检测对比;组合抗原法对12例新疆地区KS患者血清和不同地区儿童血清进行HHV-8检测,结果32例KS患者血清组合抗原ELISA与潜伏态IFA的检出效率分别为75.0%和40.6%,其中潜伏态IFA检出的13例阳性血清,12例被组合抗原ELISA成功检出。潜伏态IFA检测阴性的19例KS血清中,组合抗原检出12例阳性血清;23例肝移植后皮肤癌患者血清潜伏态IFA检测全部阴性,而组合抗原ELISA检出1例弱阳性,2方法符合率达95.6%。组合抗原对不同地区小样本血清HHV-8检测显示出不同的结果:新疆地区12例KS血清阳性率100%;儿童血清HHV-8检测结果:新疆地区儿童感染率17.8%,四川儿童1.4%,法国儿童0。结论该实验室建立的HHV-8组合抗原ELISA法在灵敏性方面较传统方法有明显提高,特异性方面差异较小,HHV-8组合抗原ELISA初步具备分子流行病学调查的灵敏性、特异性要求。

[Objective] To compare the sensitivity and specificity of combined-antigen- ELISA assay established in our lab with the standard latency-IFA in virology center of Paris, and to determine the possibility of combined- antigen-ELISA assay for epidemic study use. [Methods] 32 AIDS-KS serum and 23 serum from skin tumor patients after liver transplantation were teated HHV-8 infection with both combined-antigen-ELISA in our lab and the latency-IFA assay available in virology center of Paris, 12 serum samples from KS patients, children serum from XinJiang, Sichuan of China and France were tested by combined-antigen-ELISA in our lab. [Results] Of 32 KS, 75% and 40.6% of the serum were HHV-8 positive by combined-antigen-ELISA and the latency-IFA assay respectly. Of the 13 HHV-8 positive serum tested by the latency-IFA assay, 12 also showed positive reaction by combined-antigen-ELISA assay. 19 KS serum were HHV-8 negative by the latency-IFA assay, 12 of 19 were HHV-8 positive by combined-antigen-ELISA, of 23 skin tumor patients after liver transplantation, 19 were HHV-8 positive by combined-antigen-ELISA, and of 23 skin tumor patients after liver transplantation, 1 serum showed weak HHV-8 positive by eombined-antigen-ELISA assay and all negative by lateney-IFA assay in France. Screening serum from KS of Xinjiang and children from Xinjiang, Siehuan and France with eombined-antigen-ELISA assay showed different infection rate： KS in Xinjiang HHV-8 infection was 100%, children serum from Xinjiang, Sichuan and France were 17.8%, 1.4% and 0% respectively. [Conclusion] Compare with lateney-IFA assay, combined- antigen-ELISA assay in our lab was constructed with necessary sensitivity and specificity for HHV-8 research detection.