摘要
目的初步探讨绞股蓝多糖(PGP)对CCl4肝细胞损伤的保护作用。方法用CCl4诱肝HepG2细胞损伤,设立正常对照组、CCl4损伤组和不同浓度PGP保护组;采用倒置相差显微镜和吖啶橙(AO)荧光染色观察、四甲基偶氮唑蓝(MTT)法检测细胞活力以及流式细胞仪检测细胞周期和凋亡率的方法,观察PGP对CCl4诱导的肝细胞损伤的保护作用。结果MTT检测显示PGP保护组细胞活性明显高于损伤组,以PGP浓度为100μg/ml保护组细胞活性最高;流式细胞仪测定细胞调亡率显示PGP保护组(终浓度为100μg/ml)凋亡率明显低于CCl4损伤组,细胞周期各期细胞分布比例无明显变化。结论PGP对CCl4诱导的HepG2细胞损伤具有保护作用。
[ Objective] To investigate protective effect of gynostemma pentaphyUum makino polysaccharide （PGP） on injuring HepG2 cells. [ Methods] CCl4 was used to induce HepG2 cells injure, and the cells were divided into there groups .. normal control group , CCl4 injuring group and PGP protective group. We got the evidences from pictures taken directly and after AO staining by microscopy, and detected cell vigor, cell cycle and apoptosis rate by MTTmethod and flow cytometer respectively ,and observed the protective effect of PGP on injuring HepG2 cells. [ Results ] MTT analysis displayed that the cells activity of PGP protective groups was much higher than that of CCl4 injuring group. HepG2 cells activity was highest in 100 μg/ml PGP group. Flow cytometer analysis indicated that the apoptosis rate of 100 μg/ml PGP group was significantly lower than that of CCl4 injuring group. There was no obvious difference among different cell cycles.
出处
《山东医药》
CAS
北大核心
2007年第31期27-29,共3页
Shandong Medical Journal
基金
山东大学大学生科技创新基金项目(2005-158)。
