电针“内关”对心肌缺血再灌注损伤大鼠心肌细胞膜钠泵活性及其基因表达的影响

Effect of Electroacupuncture of "Neiguan"(PC 6) on Na^+-K^+-ATPase Activity and Its Gene Expression in Cardiocyte Membrane in Rats with Myocardial Ischemia-reperfusion Injury

目的:探讨电针"内关"对缺血再灌注损伤心肌细胞膜钠泵活性及其基因表达的影响。方法:雄性Wistar大鼠50只,随机分为假手术组、缺血再灌注模型组、电针内关组、电针神门组、电针合谷组,每组10只,针刺穴位行提插捻转手法1 min后接电针,疏密波,30/100 Hz,2~4 mA,20 min。采用冠状动脉结扎法建立心肌缺血再灌注模型。无机磷比色法测定缺血中心区心肌组织细胞膜钠泵活性,用RT-PCR法分析钠泵的基因表达。结果:缺血再灌注损伤大鼠心肌细胞膜钠泵活性降低,基因表达下调;电针"内关"在一定程度上上调钠泵的基因表达,增强其活性(P<0.01);而电针"神门""合谷"仅有上调钠泵基因表达、增强其活性的趋势,与模型组比较差异无显著性意义(P>0.05)。结论:电针"内关"可能通过上调心肌细胞膜钠泵基因表达、增强钠泵活性,以维持膜内外环境的稳定,保护心肌。

Objective： To compare the effects of electroacupuncture （EA） of ＂Neiguan＂ and other acupoints on Na^＋- K^＋ -ATPase activity and its gene expression in ischemic myocrardial cell membrane in rats. Methods： A total of 50 Wistar rats were randomly divided into pseudo-operation （control）, model, Neiguan （PC 6）, Shenmen （HT 7） and Hegu （LI 4） groups, with 10 rats in each group. Myocardial ischemia-reperfusion （I/R） model was duplicated by occlusion of the descending anterior branch of the left coronary artery and reperfusion. EA （30/100 Hz, 2-4 mA） was applied to the above-mentioned acupoints for 20 min respectively. Na^＋ -K^＋ -ATPase activity was determined by inorganic phosphorus colorimetry, and Na^＋ -K^＋ -ATPase gene expression was detected using reverse transcription polymerase chain reaction （RT-PCR） technique. Results： Compared with control group, the activity of Na^＋-K^＋-ATPase in model, PC6, HT7 and LI4 groups decreased significantly after myocardial I/R （P〈0. 05, 0.01）; while in comparison with model, the activity of Na^＋-K^＋-ATPase in PC6 group increased considerably （P〈0. 01）. Regarding the expression of Na^＋ -K^＋ -ATPase mRNA, compared with control group, it was down-regulated significantly in model, HT7 and LI4 groups （P〈0. 01）; while in PC6 group, Na^＋ -K^＋ -ATPase mRNA expression was up-regulated markedly compared with model group （ P〈0. 05） ; The activity of Na^＋ -K^＋ -ATPase and the expression of Na^＋ -K^＋ -ATPase mRNA in HT7 and LI4 groups were significantly lower than those in PC6 group （P〈0.01,0.05）. No significant differences were found between HT7 and LI4 groups in the activity of Na^＋ -K^＋ -ATPase and the expression of Na^＋ -K^＋ -ATPase mRNA （ P〉O. 05）. Conclusion： EA of ＂Neiguan＂ （PC 6） may potentiate the activity of Na^＋ -K^＋ -ATPase and up-regulate Na^＋ -K^＋-ATPase mRNA expression, which may contribute to its protective effect on ischemic cardiocytes.