点刺“委中”放血对实验性腰椎间盘突出症坐骨神经传导速度和髓核组织白细胞介素-1α的影响

Effects of Pricking Bleeding at "Weizhong"(BL 40) on Sciatic Nerve Conduction Velocity and Interleukin-1α Content in the Lumbar Vertebral Nucleus Pulposus in Experimental Lumbar Intervertebral Disc Protrusion Rabbits

目的:通过观察实验性腰椎间盘突出症(lumbar intervertebral disc protrusion,LIDP)家兔坐骨神经传导速度(sciatic nerve conduction velocity,SNCV)和髓核组织中白细胞介素-1α(interleukin-1α,IL-1α)水平变化,探讨点刺"委中"放血治疗LIDP的机制。方法:40只健康新西兰家兔随机分为4组,即空白组、LIDP模型组、点刺委中组和点刺非穴对照点组。自制的LIDP动物病理模型造模器造实验性LIDP病理模型,三棱针点刺"委中"或对照点放血治疗,左右交替,连续7 d。采用BL-410生理信号记录系统记录SNCV,酶联免疫吸附法测定腰椎间盘髓核组织中IL-1α的含量。结果:点刺委中组治疗后SNCV显著高于治疗前(P<0.01),其治疗前后SNCV的差值显著高于模型组和非穴对照组(P<0.05);点刺"委中"放血可降低LIDP家兔髓核组织中IL-1α含量,与模型组和非穴对照组比较差异有显著性意义(P<0.01,0.05)。结论:点刺"委中"穴可明显增快LIDP家兔的SNCV,减少IL-1α等炎症因子含量以减轻炎症反应可能是点刺"委中"放血治疗LIDP的机制之一。

Objective： To observe the effect of ＂Weizhong＂ （BL 40） bleeding on sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α） content of the lumbar vertebral nucleus pulposus tissue in experimental lumbar intervertebral disc protrusion （LIDP） rabbits so as to explore the underlying mechanism of blood letting in the treatment of LIDP. Methods： Forty healthy New Zealand rabbits were randomly assigned to control, model, BL40 and non-point （1 cm medial to BL 40） groups, with 10 cases in each group. LIDP model was established by pushing the intervertebral disc tissue rightward to protrude the posterior longitudinal ligament with a self-made instrument after exposing the L6-7 vertebrae and intervertebral space. Blood-letting was performed at ＂Weizhong＂ （BL 40） and non-point by using a triangle-edged needle to prick the local tissue, once daily and continuously for 7 days. SNCV was detected by using BL-410 physiological signal recording system, and the content of IL-1α in L6-7 intervertebral disc tissue determined with enzyme linked immunsorbent assay （ELISA）. Results： In comparison with control group and pre-modeling, SNCV decreased significantly in model, BL40 and non-acupoint groups after LIDP; while compared with model group, values of SNCV in BL40 group increased considerably （P〈0.05）, no significant, changes were found between non-acupoint group and model group （P〉0.05）. Compared with control group, the contents of IL-1α in the nucleus pulposus in model and non-acupoint groups increased remarkably （P〈0.05, 0.01 ）, but no significant difference was found between control and BL40 groups in IL-1α level （ P 〉 0.05）. Conclusion ： Blood-letting at ＂Weizhong＂ （BL 40） can relieve LIDP-induced nerve injury and mechanical oppression to improve SNCV , which may be closely related to its effect in decreasing inflammatory factor IL-1α level.