我国西部人群脂联素基因+45位点多态性与肥胖、胰岛素抵抗及2型糖尿病的相关性

The relationship of the adiponectin +45 nucleotide T/G polymorphism with insulin resistance ,obesity and type 2 diabetes

目的探讨宁夏汉族人群脂联素基因+45位核苷酸T/G多态性与肥胖、胰岛素抵抗(IR)及2型糖尿病(T2DM)的相关性。方法采用聚合酶链式反应一限制性内切酶长度多态性技术,对100例T2DM患者和101例正常对照(NC)者脂联素基因+45位点进行基因分型;并计算BMI和HOMA-IR。结果(1)T2DM组GG基因型频率明显高于NC组(P<0.01),G等位基因频率明显高于NC组(P<0.01)。(2)在T2DM组中,GG+TG基因型的BMI、HOMA-IR大于TT基因型(P<0.01)。在NC组中,各基因型间BMI、HOMA-IR的差异无统计学意义。在T2DM组中,而BMI≥25组的GG+TG基因型频率高于BMI<25组(P<0.01),G等位基因频率也高于BMI<25组(P<0.01)。结论脂联素基因+45位核苷酸T/G多态性与肥胖、IR及T2DM相关。

Objective To study the relationship of the adiponectin ＋ 45 T/G polymorphism with in- sulin resistance ,obesity and type 2 diabetes. Methods The 100 type 2 diabetics and 101 healthy subjects with normal glucose tolerance were enrolled. All subjects were genotyped for the polymorphism of adiponeetin by PCR-RFLP assay. Body mass index （BMI） and insulin resistance index （HOMA-IR） were evaluated. Results （ 1 ） The frequency of GG genotype was higher in type 2 diabetic group than in normal controls （ P 〈 0.01 ）. The frequency of G allele was higher in type 2 diabetic group than in normal controls（ P 〈 0.01 ）. （2） The BMI and HOMA - IR in the type 2 diabetic group was higher in TG ＋ GG genotype than in TF geno- type（ P 〈 0.01 ）. The frequencies of TG ＋ GG genotype and G allele of the type 2 diabetic group were higher in BMI≥25 subgroup than in BMI 〈 25 subgroup（ P 〈 0.01 ）. Conclusion The polymorphism of adiponeetin ＋45 nueleotide T/G is associated with obesity,insulin resistance, and type 2 diabetes.