男女腹部皮下和网膜脂肪中脂联素、肿瘤坏死因子α、过氧化物酶体增殖物激活受体γ_2 mRNA表达的比较

The comparison of mRNA expressions of adiponectin,tumour necrosis factor alphal and peroxisomal proliferator-activated receptorγ_2 between male and female in human abdominal subcutaneous and omental adipose tissue

目的比较男女腹部皮下脂肪组织、网膜脂肪组织中脂联素、肿瘤坏死因子-a（TNF-a）、过氧化物酶体增殖物激活受体γ2（PPARγ2）mRNA表达。方法选择30例择期外科手术患者，男15例，女15例，用RT-PCR法检测腹部皮下脂肪组织和网膜脂肪组织中脂联素、TNF-a、PPARγ2 mRNA表达。结果（1）男性皮下脂肪组织中脂联素mRNA显著低于女性，网膜脂肪组织中TNF-a mRNA显著高于女性（均P〈0．05）。（2）女性皮下脂肪组织中脂联素mRNA表达高于其网膜脂肪组织，男女网膜脂肪组织中TNF-a mRNA均高于各自的皮下脂肪组织（P〈0．05～0．01）。（3）男女间皮下脂肪组织和网膜脂肪组织中PPARγ2 mRNA表达差异无统计学意义（P〉0．05）。结论人腹部皮下脂肪组织和网膜脂肪组织中脂联素、TNF-a mRNA的表达可能存在性别差异，而PPARγ2 mRNA表达的性别差异则不明显。

Objective To investigate the differences of mRNA expressions of adiponectin（APN） ,tumour necrosis factor alphal（TNF-α） and peroxisomal proliferator-activated receptor,/2 （PPARγ2） between male and fe- male in human abdominal subcutaneous and omental adipose tissue. Methods 30 patients to be operated were divided into male group（n = 15） and female group（n = 15）. The mRNA expressions of APN ,TNF-α and PPARγ2 in abdominal subcutaneous and omental adipose tissue were measured using RT-PCR method. Results （ 1 ） APN mRNA expression in subcutaneous adipose tissue was lower in male than in female, meanwhile TNF-α mRNA expression in omental adipose tissue was higher in male than in female （all P 〈 0.05）. （2） APN mRNA expression in subcutaneous adipose tissue of female was higher than that in omental adipose tissue. No matter in male and in female group, TNF-α mRNA expression was higher in omental adipose tissue than in subcutaneous adipose tissue （P 〈 0, 05 - 0.01 ）. （3） No significant differences between male and female groups were found for PPARγ2 mRNA expression in both subcutaneous and omental adipose tissue （P 〉 0.05）. Conclusions There might be a signifi- cant difference in mRNA expressions of APN and TNF-α, except for PPARγ2 mRNA expression , between male and female in human abdominal subcutaneous and omental adipose tissue.