曲尼司特对小鼠变应性接触性皮炎的体外研究

In vitro study of tranilast on allergic contact dermatitis in mice

目的:研究曲尼司特对变应性接触性皮炎的抑制作用,并探讨其作用机制。方法:建立小鼠变应性接触性皮炎(allergic contact dermatitis,ACD)模型,体外法测定曲尼司特各剂量对小鼠脾细胞培养上清液中白介素-2(IL-2)、干扰素-γ(IFN-γ)水平的影响。结果:曲尼司特给药浓度大于等于30μg/mL各组可剂量依赖性抑制上清液中IL-2、IFN-γ水平,差异有统计学意义(P<0.01)。结论:曲尼司特可能通过抑制IL-2、IFN-γ水平在变应性接触性皮炎治疗中发挥作用。

Aim： To investigate the mechanism of inhibition of tranilast on allergic contact dermatitis. Methods： The changes in levels of IL-2 and IFN-7 in the culture supernatant of spleen cell by giving different doses of tranilast at various times in vitro were observed in the murine model of allergic contact dermatitis. Results： The levels of IL-2 and IFN-7 were dose-dependently suppressed in groups of tranilast（ ≥30 μg/mL）, and the difference had statistical significance （ P 〈 0.01 ）. Conclusion： The therapeutic effect of tranilast in allergic contact dermatitis is possibly played by inhibiting the levels of IL-2 and IFN-γ.