抗晚疫病马铃薯的花药培养研究

Anther culture of late blight resistant potato

对4个品种的抗晚疫病马铃薯进行了花药培养的研究。结果表明:不同培养基对同一品种花药的总诱导数和胚状体诱导数有显著影响;在相同培养基条件下,不同品种的总诱导数差异显著,胚状体诱导数差异不显著;总诱导率最高的诱导培养基是D:MS+2,4-D2 mg/L+BAP2 mg/L+活性炭2 g/L+马铃薯块茎提取液200 g/L;适合S17、T3、KW47诱导胚状体的培养基是B:MS+NAA2 mg/L+BAP2 mg/L+活性炭2 g/L+马铃薯块茎提取液200 g/L。

Anthers of four late blight resistant potato varieties were cultured. The results indicated that various culture medium had significant differences in total inducement and embryo inducement of the same potato, the total inducement was different in the four varieties in the same culture medium, the best medium of inducement was MS ＋ 2,4 - D2 mg/L＋ BAP2 mg/L＋ AC2 g/L＋ 200 g/L with distilled juice of potato tuber,S17,T3 and Kw47 were induced easily to form embryo on MS＋ NAA2 mg/L＋ BAP2 mg/L＋ AC2 g/L ＋ 200 g/L with distilled juice of potato tuber.