摘要
Sedum alfredii Hance, a newly discovered hyperaccumulator, could serve as a good material for phytoremediation of Cd polluted sites. Malondialdehyde （MDA）, reactive oxygen species （ROS） and antioxidases （catalase （CAT）; superoxide dismutase （SOD）; peroxidase （POD）） in the leaf were determined when S. alfredii was treated for 15 d with various CdC12 concentrations ranging from 0 to 800 μmol/L. The results showed that the production rate of 2＇,7＇-dichlorofluorescein （DCF）, which is an indicator of ROS level, reached up to the maximum at 400 μmol/L CdCl2 and then declined with the increase of CdCl2 concentration, while MDA accumulation tended to increase. CAT activity was significantly inhibited at all tested CdCl2 concentrations and SOD activity was sharply suppressed at 800 μmol/L CdCl2. However, the enhancement of POD activity was observed when CdCl2 concentration was higher than 400 μmol/L. In addition, its activity increased when treated with 600 μmol/L CdCl2 for more than 5 d. When sodium benzoate, a free radical scavenger, was added, S. alfredii was a little more sensitive to Cd toxicity than that exposed to Cd alone, and the Cd accumulation tended to decline with the increase of sodium benzoate concentration. It came to the conclusions that POD played an important role during Cd hyperaccumulation, and the accumulation of ROS induced by Cd treatment might be involved in Cd hyperaccumulation.
Sedum alfredii Hance, a newly discovered hyperaccumulator, could serve as a good material for phytoremediation of Cd polluted sites. Malondialdehyde （MDA）, reactive oxygen species （ROS） and antioxidases （catalase （CAT）; superoxide dismutase （SOD）; peroxidase （POD）） in the leaf were determined when S. alfredii was treated for 15 d with various CdC12 concentrations ranging from 0 to 800 μmol/L. The results showed that the production rate of 2＇,7＇-dichlorofluorescein （DCF）, which is an indicator of ROS level, reached up to the maximum at 400 μmol/L CdCl2 and then declined with the increase of CdCl2 concentration, while MDA accumulation tended to increase. CAT activity was significantly inhibited at all tested CdCl2 concentrations and SOD activity was sharply suppressed at 800 μmol/L CdCl2. However, the enhancement of POD activity was observed when CdCl2 concentration was higher than 400 μmol/L. In addition, its activity increased when treated with 600 μmol/L CdCl2 for more than 5 d. When sodium benzoate, a free radical scavenger, was added, S. alfredii was a little more sensitive to Cd toxicity than that exposed to Cd alone, and the Cd accumulation tended to decline with the increase of sodium benzoate concentration. It came to the conclusions that POD played an important role during Cd hyperaccumulation, and the accumulation of ROS induced by Cd treatment might be involved in Cd hyperaccumulation.
基金
Project supported by the Wuhan Chenguang Project for Youth Scholar(No.20045006071-24)
"211 Project"of Educational Ministry of China
