茶树菇提取物抗氧化性的研究

Study on the Antioxidation of Agrocybe cylindracea Extracts

为更好地开发利用茶树菇。采用氮蓝四唑(NBT)光化还原法、抗坏血酸-Cu2+-H2O2体系法和亚油酸体系法,研究茶树菇提取物清除超氧自由基O2-.、羟基自由基.OH和脂过氧自由基ROO.的效果。在配制的不同质量浓度的茶树菇提取物溶液中,在一定的浓度范围内,茶树菇提取物对超氧自由基O2-.、羟基自由基.OH和脂过氧自由基ROO.的清除率随着浓度的升高呈上升趋势。质量浓度分别为17.78、4.67、12.82 mg/ml时,茶树菇提取物对O2-.、ROO.和.OH的清除率最高,依次为69.29%、63.59%、90.26%。茶树菇提取物有较强的抗氧化能力,作为天然的抗氧化剂具有良好的开发前景。

The aim of the research was to develop and utilize Agrocybe cylindracea better.NBT photochemical reduction method,ascorbic acid-Cu2＋-H2O2 system method and linoleic acid system method were used to study the scavenging effect of A.cylindracea extracts on O2-·,·OH and ROO·.Among prepared A.cylindracea extract solutions with different mass concentrations,in certain concentration range,the scavenging rate of A.cylindracea extracts on O2-·,·OH and ROO· showed an increasing trend with the rise of concentration.When the mass concentrations were 17.78,4.67,12.82 mg/ml respectively,the scavenging rates of A.cylindracea extracts on O2-·,·OH and ROO· were highest,being 69.29%,63.59% and 90.26% respectively.A.cylindracea extracts had strong antioxidation capability,which had good development foreground as nature antioxidant.