摘要
通过对秀珍菇原生质体的分离与再生的影响因素的试验研究,表明以0.6M甘露醇为渗稳剂用1%纤维素酶+1%蜗牛酶,30℃酶解3～4小时原生质体产生最高,原生质体再生则以0.6M甘露醇或0.6M蔗糖为渗稳剂较好。
Through the experiments on the facts which affect the isolation and regeneration of Pleurotus comucopiae （Paul.：Pers.）Rolland protoplast, the result shows that the rate of protoplast was produced to the top by means of osmotic stabilizer of 0.6 M annitol, 1% cellulose and 1% novozyme, after 3-4 hours with 30℃. And it turns out that 0.6 annitol or 0.6 sucrose is used as osmotic stabilizer to make protoplast regeneration.
出处
《岳阳职业技术学院学报》
2006年第5期69-70,共2页
Journal of Yueyang Vocational and Technical College
关键词
秀珍菇
原生质体
分离
再生
pleurotus cornucopiae （Paul.：Pers.） rolland
protoplast
isolation
regeneration
