游离丙型肝炎病毒核心抗原检测的临床价值探讨

Clinical value of the detection of hepatitis C virus core antigen

目的:探讨游离丙型肝炎病毒(HCV)核心抗原检测在HCV感染诊断中的价值。方法:采用荧光定量PCR法检测HCV-RNA、ELISA法同步检测抗-HCV和游离HCV核心抗原。结果:191例HCV感染者HCV-RNA的检出率为71·2%(136/191);抗-HCV的检出率为97·4%(186/191);游离HCV核心抗原的检出率为33·0%(63/191)。其中有2例经抗病毒治疗的患者HCV-RNA和抗-HCV检测均阴性,但游离HCV核心抗原检测阳性;另有1例患者抗-HCV阴性,而游离HCV核心抗原阳性,经HCV-RNA证实为HCV感染。27例非HCV感染者HCV-RNA、抗-HCV和游离HCV核心抗原检测结果均为阴性。结论:HCV核心抗原检测作为抗-HCV检验的补充试验对HCV感染的诊断具有重要价值。

AIM： To explore the value of detecting hepatitis C virus core antigen in HCV infected diseases. METHODS： The HCV-RNA was tested by fluorescent quantitative polymerase chain reaction （FQ-PCR）. The core antigens of HCV and anti-HCV were tested by enzyme-linked immunosorbent assay（ELISA）. RESULTS： The positive rate of HCV-RNA in 191 patients was 71. 2%, about 136 in 191. The positive rate of anti-HCV was 97.4%, about 186 in 191. The positive rate of HCV core antigen was 33.0%, about 63 in 191. After antiviral therapy for 2 patients,their HCV-RNA and anti-HCV were detected negative but their HCV core antigen was detected positive. In another patient, his anti- HCV was negative but his HCV core antigen was positive. CONCLUSION： As a supplemental test for anti-HCV exami- nation the detection of HCV core antigen is of important clinical value in HCV infection diagnosis.