hTERT启动子驱动的TRAIL诱导宫颈癌细胞的凋亡作用

hTERT promoter regulated tumor targeting TRALL in cervical cancer cell

目的:探讨端粒酶(hTERT)启动子驱动的肿瘤坏死因子相关凋亡诱导配体(TRAIL)对宫颈癌细胞生长抑制的作用。方法:脂质体转染将hTERT-TRAIL转染至宫颈癌细胞HeLa,RT-PCR检测TRAILmRNA的表达;通过MTT、流式细胞术检测稳定转染细胞的生长情况,电镜观察转染细胞的超微结构。结果:转染真核表达载体hTERT-TRAIL组细胞中TRAILmRNA细胞表达量高于对照组(P<0.05);hTERT-TRAIL组细胞对宫颈癌细胞的抑制率高于CMV-TRAIL组和对照组(P<0.05);hTERT-TRAIL组细胞对宫颈癌细胞的凋亡率明显高于转染CMV-TRAIL组和对照组(P<0.01);电镜结果显示,hTERT-TRAIL对细胞作用是以细胞凋亡为主。结论:hTERT启动子驱动的肿瘤坏死因子相关凋亡诱导配体对人宫颈癌细胞的生长有明显的抑制作用和诱导凋亡作用,为宫颈癌的研究奠定基础和临床治疗提供思路。

AIM： To investigate the effect of hTERT promoter which regulated tumor targeting TRALL on the cervical cancer cell line HeLa. METHODS： The mRNA expression of TRAIL on HeLa was examined by RT-PCR. The proliferation, apoptosis, motion of the transfected cervical cancer cell were detected by MTT, flow cytometry, and cell invasion assay respectively. The ultrastructure was observed by electron microscope. RESULTS： Compared with the control group, the expression of TRAILmRNA was significantly upregulated in hTERT-TRAIL （P 〈0.05）. The apotosis rate and the inhibitory rate of cell growth of hTERTTRAIL was significantly high （ P 〈 0.05 ）. Electron microscope results indicated that hTERT promoter regulated tumor targeting TRALL facilitated the apoptosis of HeLa. CONCLUSION： The hTERT-TRAIL significantly inhibits the malignant proliferation and invasion ability of the cervical cancer cell line, and facilitates the apoptosisof HeLa, which lays a foundation for the treatment of patients with cervical cancer.