RP-HPLC测定茅苍术中β-桉叶醇的含量

Determination of β-eudesmol in rhizome of Atractylodes lancea by RP-HPLC

目的：采用RP—HPLC测定茅苍术中β-桉叶醇的含量，为茅苍术质量标准研究提供依据。方法：采用反相高效液相色谱法，色谱柱为Inertsil ODS-3（4．6mm×250mm，5μm）；流动相乙腈-水（68：32）；流速1．0mL·min^-1；检测波长200nm；柱温为25℃。结果：测得10批茅苍术药材中β-桉叶醇含量为0．833～4．466mg·g^-1，β-桉叶醇为0．048～1．200μg，r=0．9999，平均回收率99．3％，RSD1．4％（n=9）。结论：建立了高效液相色谱法测定茅苍术中β-桉叶醇含量的方法，该方法准确，可靠，可用于茅苍术药材的测定。

Objective： To develop a RP-HPLC method for determination ofβ-eudesmol in rhizome ofAtractylodes lancea, and to provide valuble data for quality control of A. lancea. Method： The samples were separated on an Inertsil ODS-3 （ 4. 6 mm × 250 mm,5 μm） column with the mobile phase of acetonitrile-water（ 68： 32）. Flow rate was 1.0 mL · min^-1. The detection wavelength was set at 200 nm. Column temperature was 25 ℃. Result： The contents ofβ-eudesmol determinated was 0. 833-4. 466 mg ·g^-1 ,The linear range of β-eudesmol was 0. 048-1. 200μg（r =0. 999 9） ,the average recovery was 99.3% ,RSD was 1.4% （n =9）. Conclusion： The method for quantitation ofβ-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea.