通心络对气虚和气滞型血管内皮损伤相关基因表达谱的影响

Effect of Tongxinluo on 7 gene expression profile associated with vascular endothelium injure of rats with deficiency of vital energy or qi stagnation

目的:探讨气虚和气滞型血管内皮损伤相关的炎症、氧化应激等7组基因表达谱的异同,及通心络对基因表达谱的影响。方法:用高L-蛋氨酸复制SD大鼠血管内皮损伤,并附以负重游泳法和束缚法分别制造气虚和气滞证实验模型。应用RT-PCR技术和NCB I提供的基因表达系列分析(SAGE)数据库及在线工具,分析气虚和气滞型内皮损伤相关的炎症、氧化应激等7组基因表达谱的变化,以及通心络对基因表达谱的影响。结果:与正常组相比,气虚组炎症相关环氧合酶-1(COX-1)、环氧合酶-2(COX-2),氧化应激相关诱导型一氧化氮合酶(iNOS)、超氧化物歧化酶(SOD)及血管舒缩相关内皮型一氧化氮合酶(eNOS)、内皮素转换酶(ECE)基因的表达均显著升高(P<0.05或P<0.01),前列环素合酶(PCS)基因表达变化无统计学差异,通心络治疗后这些基因的表达降低(P<0.05或P<0.01);气滞组COX-1,COX-2,iNOS及eNOS,ECE基因表达亦显著升高(P<0.01),PCS和SOD基因表达显著下降(P<0.01),通心络治疗后这些基因的异常表达趋于正常(P<0.01)。结论:气虚和气滞型血管病变中,内皮损伤7组相关基因表达谱不尽相同,而通心络能改善这些基因表达谱的异常,从而起到保护血管内皮免受损伤的作用。

Objective： To investigate 7 gene expression profile associated with inflammation and oxidative stress in vascular endothelium injure of rats with deficiency of vital energy or qi stagnation, and the effect of Tongxinluo on gene expression profile. Method： The model of vascular endothelium injury of rats with deficiency of vital energy or qi stagnation were established by using high L- methionine, with load-carrying swimming or being fastened, respectively. RT-PCR and SAGE database which is available in NCBI, were used to analyze the changes of 7 gene expression related with inflammation and oxidative stress in endothelium injure and the effect of Tongxinluo on the gene expression profile. Result： Compared with control group, the gene expression of inflammation related COX- 1, COX-2, oxidative stress related iNOS, SOD and blood vessel vasomotion related eNOS, ECE, increased in deficiency of vital energy group （ P 〈 0. 05 or P 〈 0. 01 ）, and the gene expression decreased with Tongxinluo treatment （ P 〈 0. 05 or P 〈 0. 01 ）. The gene expression of COX-1, COX-2, iNOS and eNOS, ECE, increased （P 〈 0. 01 ）, but the gene expression of PCS and SOD decreased （P 〈 0. 01 ）, in qi stagnation group, and the disorder of gene expression improved with treatment of Tongxinluo （P 〈 0. 01 ）. Conclusion： The 7 gene expression related to vascular endothelium injure were not the same in rat with deficiency of vital energy or qi stagnation, and Tongxinluo could regulate the disorder of the gene expression, protecting vascular endothelium from injure.