黄连解毒汤对快速老化模型小鼠海马蛋白表达的影响

Effect of Huanglian Jiedutang on expression of hippocampus proteomics in senescence accelerated mouse

目的:研究黄连解毒汤(HLJDT)对快速老化模型小鼠(senescence accelerated mouse,SAM)的快速老化亚系SAM-prone/8(SAMP8)海马蛋白表达的影响,以期从分子水平较深入地阐明HLJDT治疗阿尔茨海默病(AD)的机制。方法:将12月龄SAM的快速老化亚系SAMP8和抗快速老化亚系SAM-resistance/1(SAMR1)分为SAMR1对照组、SAMP8模型组和HLJDT给药组,应用双向凝胶电泳结合质谱鉴定,观察HLJDT对SAM海马组织蛋白表达的影响。结果:与SAMR1相比,SAMP8海马中有29个蛋白点的表达发生显著变化;给予HLJDT后有38个蛋白点的表达发生显著变化。选取部分HLJDT的作用蛋白点进行质谱分析结合数据库检索,共鉴定出12个蛋白,这些蛋白涉及到能量代谢、转录调控、细胞骨架、氨基酸代谢等功能。其中有4个蛋白是SAMP8与SAMR1海马蛋白比较存在显著差异的蛋白,给予HLJDT后其蛋白表达得到了明显改善。结论:HLJDT通过调节SAMP8脑中与能量代谢、信号转导、细胞骨架及氨基酸代谢等相关蛋白的表达,从多途径对SAMP8的老化起到了改善作用。

Objective： To investigate the effect of Huanglian Jiedutang （HLJDT） on hippocampal protein expressions in senes- cence accelerated mouse-prone/8 （SAMP8）. Method： The 12-month-old senescence accelerated mice （SAM） were divided into three groups： SAM-resistance/1 （SAMR1）, SAM-prone/8 （SAMP8） and SAMP8 treated with HLJDT. The effect of HLJDT on expressions of hippocampal proteins was analyzed by two dimensional electrophoresis （2DE） and matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）. Result： Compared with same age SAMR1, there were 29 differential expressed hippocampal proteins in SAMP8. After treated with HLJDT, the expressions of 38 hippocampal proteins of SAMP8 were changed significantly. 12 reactive proteins of HLJDT were chosen to be identified by MALDI-TOF-MS and the results were searched in MASCOT database. Among 12 reactive proteins, the expressions of 4 hippocampal proteins which expressed differentially between SAMR1 and SAMP8 could be improved by HLJDT. Conclusion： HLJDT may improve the aging of SAMP8 by regulating the expressions of proteins related with energy metabolism, signal transduction, cytoskeletal, amino acid metabolism and so on.